Product nameExtraction Buffer 5X PTR
Abcam’s Extraction Buffer 5X is formulated for the preparation of cell or tissues extracts for use in immunoassays.
The Extraction Buffer should be diluted to 1X in the appropriate buffer before use. If required the Extraction Buffer 5X can be used in conjunction of Abcam’s Extraction Enhancer Buffer 50X (ab193971).
Instructions for use
Before use the Extraction Buffer 5X should be diluted to 1X with distilled water.
Note: The provided Extraction Buffer 5X contains phosphatase inhibitors and protease inhibitor aprotinin. Additional protease inhibitors can be added if required.
If the Extraction Buffer 5X is to be used in conjunction with Extraction Enhancer Buffer 50X (ab193971), then follow the instructions below:
1X Extraction Buffer + Enhancer
Prepare 1X Extraction Buffer + Enhancer by diluting Extraction Buffer 5X and Extraction Enhancer Buffer 50X to 1X with deionized water. To make 10 mL 1X Extraction Buffer + Enhancer combine 7.8 mL deionized water, 2 mL Extraction Buffer 5X and 200 µL Extraction Enhancer Buffer 50X. Mix thoroughly and gently.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab193970 has been referenced in 16 publications.
- Kaneshige A et al. Relayed signaling between mesenchymal progenitors and muscle stem cells ensures adaptive stem cell response to increased mechanical load. Cell Stem Cell 29:265-280.e6 (2022). PubMed: 34856120
- Beyersdorf JP et al. Robust, Durable Gene Activation In Vivo via mRNA-Encoded Activators. ACS Nano 16:5660-5671 (2022). PubMed: 35357116
- Kemp KC et al. An open-label pilot study of recombinant granulocyte-colony stimulating factor in Friedreich's ataxia. Nat Commun 13:4655 (2022). PubMed: 35945193
- Liu H et al. Reducing Akt2 in retinal pigment epithelial cells causes a compensatory increase in Akt1 and attenuates diabetic retinopathy. Nat Commun 13:6045 (2022). PubMed: 36229454
- Delvaux M et al. Ferroptosis Induction and YAP Inhibition as New Therapeutic Targets in Gastrointestinal Stromal Tumors (GISTs). Cancers (Basel) 14:N/A (2022). PubMed: 36291834