For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/sample-preparation-kits/extraction-buffer-5x-ptr-ab193970.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Tools and Reagents Extraction
Share by email
SimpleStep

Extraction Buffer 5X PTR (ab193970)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

You may also be interested in

ChIP
Product image
ChIP-Seq High Sensitivity Kit (ab185908)
ELISA
Product image
Human BDNF ELISA Kit (ab212166)

View more associated products

Overview

  • Product name

    Extraction Buffer 5X PTR
  • Product overview

    Abcam’s Extraction Buffer 5X is formulated for the preparation of cell or tissues extracts for use in immunoassays.


    The Extraction Buffer should be diluted to 1X in the appropriate buffer before use. If required the Extraction Buffer 5X can be used in conjunction of Abcam’s Extraction Enhancer Buffer 50X (ab193971).

  • Notes

    Instructions for use

    Before use the Extraction Buffer 5X should be diluted to 1X with distilled water. 

    Note: The provided Extraction Buffer 5X contains phosphatase inhibitors and protease inhibitor aprotinin. Additional protease inhibitors can be added if required.

    If the Extraction Buffer 5X is to be used in conjunction with Extraction Enhancer Buffer 50X (ab193971), then follow the instructions below:

    1X Extraction Buffer + Enhancer

    Prepare 1X Extraction Buffer + Enhancer by diluting Extraction Buffer 5X and Extraction Enhancer Buffer 50X  to 1X with deionized water. To make 10 mL 1X Extraction Buffer + Enhancer combine 7.8 mL deionized water, 2 mL Extraction Buffer 5X and 200 µL Extraction Enhancer Buffer 50X.  Mix thoroughly and gently.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 10 ml 50 ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 50ml
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Extraction

Associated products

  • Related Products

    • Extraction Enhancer Buffer 50X (ab193971)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab193970? Please let us know so that we can cite the reference in this datasheet.

ab193970 has been referenced in 10 publications.

  • Ghosh S  et al. ßA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity. Commun Biol 4:248 (2021). PubMed: 33627831
  • Van den Broek B  et al. Microglial derived extracellular vesicles activate autophagy and mediate multi-target signaling to maintain cellular homeostasis. J Extracell Vesicles 10:e12022 (2020). PubMed: 33708355
  • Solti K  et al. DJ-1 can form ß-sheet structured aggregates that co-localize with pathological amyloid deposits. Neurobiol Dis 134:104629 (2020). PubMed: 31669752
  • Montaser A  et al. L-Type Amino Acid Transporter 1-Utilizing Prodrugs of Ketoprofen Can Efficiently Reduce Brain Prostaglandin Levels. Pharmaceutics 12:N/A (2020). PubMed: 32290494
  • Montalvo-Parra MD  et al. Experimental design of a culture approach for corneal endothelial cells of New Zealand white rabbit. Heliyon 6:e05178 (2020). PubMed: 33072921
  • Hosseinkhani B  et al. (Sub)populations of extracellular vesicles released by TNF-a -triggered human endothelial cells promote vascular inflammation and monocyte migration. J Extracell Vesicles 9:1801153 (2020). PubMed: 32944190
  • Fragniere AMC  et al. Hyperosmotic stress induces cell-dependent aggregation of a-synuclein. Sci Rep 9:2288 (2019). PubMed: 30783136
  • Ghosh S  et al. Neutrophils homing into the retina trigger pathology in early age-related macular degeneration. Commun Biol 2:348 (2019). PubMed: 31552301
  • Hosseinkhani B  et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). PubMed: 30131806
  • Ryan ZC  et al. 1a,25-Dihydroxyvitamin D3 Regulates Mitochondrial Oxygen Consumption and Dynamics in Human Skeletal Muscle Cells. J Biol Chem 291:1514-28 (2016). PubMed: 26601949

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Inquiry: I will be employing your extraction buffer, which is provided in your ELISA kit, to prepare a cell lysate from a cell population.
Thereafter, i would like to quantify the total protein concentration in the cell lysate in order to normalize the concentration between samples. Does this buffer interfere with conventional protein concentration assays (Bradford or other)? In other words, will I obtain a false positive due to the buffer or the protein inhibitors present within the buffer? If so, how can I quantify my lysate after using this buffer?

Read More

Abcam community

Verified customer

Asked on Feb 02 2015

Answer



I can confirm that the extraction buffer is Triton X-100 based. It does not interfere with BCA protein method at 5X or 1X concentrations. Commercial Bradford assay specifications list Triton as interfering with the assay at 0.05%. Therefore 1X extraction buffer should be diluted at least 5-fold to be compatible with the Bradford method.

I suggest to add an extraction buffer only blank as a control measurement in your experiment.

Read More

Anja Hoffmann

Abcam Scientific Support

Answered on Feb 02 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.