Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
Overview
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Product name
Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) -
Product overview
Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.
The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.
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Notes
The kit protocol is very simple:
- incubate cells with Fixation Medium for 15 min
- add PBS and spin down cells
- add Permeabilization Medium and antibody to cell pellet
- incubate for 15 min
- wash cells with PBS and analyze
The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).
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Tested applications
Suitable for: Flow Cytmore details
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 50 tests 200 tests Fixation Medium (Reagent A) 1 x 5ml 4 x 5ml Permeabilization Medium (Reagent B) 1 x 5ml 4 x 5ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
Images
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Normal blood staining with Fix and Perm
Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).
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Normal blood staining with Fix and PermDouble labeling of a normal blood sample treated with ab185917, and immunostained for CD3 (PE) and MPO-C2 (FITC).
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Normal blood staining with Fix and PermDouble labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and MPO-C2 (FITC).
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Normal blood staining with Fix and PermForward and side scatter pattern of a normal blood sample treated with ab185917.
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MPO staining with Fix and PermImmunostaining with anti MPO-FITC conjugate of undifferentiated leukemia cells of AML type.
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CD3 staining with Fix and PermImmunostaining with CD3-PE conjugate of surface CD3-negative undifferentiated leukemia cells of T-ALL type.
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CD22 staining with Fix and PermImmunostaining with CD22-PE conjugate of undifferentiated leukemia cells of B-ALL type.
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Intracellular MPO and Lactoferrin staining with Fix and PermFlow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.
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Leukemia cell staining with Fix and PermFlow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using ab185917, followed by immunostaining for lactoferrin and MPO-C2.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab185917 has been referenced in 6 publications.
- Baek MO et al. Self-transducible LRS-UNE-L peptide enhances muscle regeneration. J Cachexia Sarcopenia Muscle 13:1277-1288 (2022). PubMed: 35178893
- Sangree AK et al. Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2. Nat Commun 13:1318 (2022). PubMed: 35288574
- Li D et al. Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging. Theranostics 11:5585-5604 (2021). PubMed: 33859765
- Wu J et al. Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke. Aging Dis 12:786-800 (2021). PubMed: 34094642
- Aligholipour Farzani T et al. Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models. Vaccines (Basel) 7:N/A (2019). PubMed: 31527460
- Deng YP et al. Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells. Exp Ther Med 14:753-758 (2017). PubMed: 28672995