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    products/sample-preparation-kits/fix--perm--cellfixation--permeabilization-kit-flow-cytometry-ab185917.pdf

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Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (3)

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Normal blood staining with Fix and Perm
  • Normal blood staining with Fix and Perm
  • Normal blood staining with Fix and Perm
  • Normal blood staining with Fix and Perm
  • MPO staining with Fix and Perm
  • CD3 staining with Fix and Perm
  • CD22 staining with Fix and Perm
  • Intracellular MPO and Lactoferrin staining with Fix and Perm
  • Leukemia cell staining with Fix and Perm

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Overview

  • Product name

    Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry)
  • Product overview

    Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.


    The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.

  • Notes

    The kit protocol is very simple:

    - incubate cells with Fixation Medium for 15 min

    - add PBS and spin down cells

    - add Permeabilization Medium and antibody to cell pellet

    - incubate for 15 min

    - wash cells with PBS and analyze

    The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).

  • Tested applications

    Suitable for: Flow Cytmore details

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 50 tests 200 tests
    Fixation Medium (Reagent A) 1 x 5ml 4 x 5ml
    Permeabilization Medium (Reagent B) 1 x 5ml 4 x 5ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Molecular Biology Kits
    • Permeabilization

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
Notes
Flow Cyt
Use at an assay dependent concentration.

Images

  • Normal blood staining with Fix and Perm
    Normal blood staining with Fix and Perm

    Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).

  • Normal blood staining with Fix and Perm
    Normal blood staining with Fix and Perm

    Double labeling of a normal blood sample treated with ab185917, and immunostained for CD3 (PE) and MPO-C2 (FITC).

  • Normal blood staining with Fix and Perm
    Normal blood staining with Fix and Perm

    Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and MPO-C2 (FITC).

  • Normal blood staining with Fix and Perm
    Normal blood staining with Fix and Perm

    Forward and side scatter pattern of a normal blood sample treated with ab185917.

  • MPO staining with Fix and Perm
    MPO staining with Fix and Perm

    Immunostaining with anti MPO-FITC conjugate of undifferentiated leukemia cells of AML type.

  • CD3 staining with Fix and Perm
    CD3 staining with Fix and Perm

    Immunostaining with CD3-PE conjugate of surface CD3-negative undifferentiated leukemia cells of T-ALL type.

  • CD22 staining with Fix and Perm
    CD22 staining with Fix and Perm

    Immunostaining with CD22-PE conjugate of undifferentiated leukemia cells of B-ALL type.

  • Intracellular MPO and Lactoferrin staining with Fix and Perm
    Intracellular MPO and Lactoferrin staining with Fix and Perm

    Flow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.

  • Leukemia cell staining with Fix and Perm
    Leukemia cell staining with Fix and Perm

    Flow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using ab185917, followed by immunostaining for lactoferrin and MPO-C2.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab185917? Please let us know so that we can cite the reference in this datasheet.

ab185917 has been referenced in 3 publications.

  • Li D  et al. Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging. Theranostics 11:5585-5604 (2021). PubMed: 33859765
  • Aligholipour Farzani T  et al. Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models. Vaccines (Basel) 7:N/A (2019). PubMed: 31527460
  • Deng YP  et al. Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells. Exp Ther Med 14:753-758 (2017). PubMed: 28672995

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