HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control (ab136806)
Overview
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Product name
HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control -
Product overview
Staurosporine is a broad-spectrum inhibitor of serine/threonine kinases and tyrosine kinases. It is commonly used to induce apoptosis in experimental settings, although the mechanism is not fully understood.
The staurosporine-treated HeLa lysate is designed for use as a western blot positive control for antibodies against apoptosis-specific proteins or for experimental treatments to induce apoptosis. -
Notes
Confluent HeLa cells were treated with either the vehicle (DMSO, 0.2%) or staurosporine (1 uM) for 4 hours. Cells were pelleted then lysed with Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM NaF, 1x Protease Inhibitors, 1% SDS) and the lysate cleared by sonication. The protein concentration was measured by BCA assay. Modified 5x SDS sample buffer was added to the lysate before storage at -80ºC. The lysates were validated by western blotting with the ab136812 Apoptosis WB cocktail (cleaved PARP, actin, caspase 3).
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Tested applications
Suitable for: WB, SDS-PAGEmore details
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 2 units Staurosporine-Treated HeLa Lysate 1 x 200µg Control for Staurosporine-Treated Lysate 1 x 200µg -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
Suggested working concentration: 2.0 mg/mL, Load 20 µg/lane. User may choose to boil the sample before loading, at their discretion. |
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| SDS-PAGE |
Use at an assay dependent concentration.
Suggested working concentration: 2.0 mg/mL, Load 20µg/lane. User may choose to boil the sample before loading, at their discretion. |
| Notes |
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WB
Use at an assay dependent concentration. Suggested working concentration: 2.0 mg/mL, Load 20 µg/lane. User may choose to boil the sample before loading, at their discretion. |
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SDS-PAGE
Use at an assay dependent concentration. Suggested working concentration: 2.0 mg/mL, Load 20µg/lane. User may choose to boil the sample before loading, at their discretion. |
Images
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Western BlotLane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 µM staurpsorine, 4 hours Load 20 µg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab136806 has not yet been referenced specifically in any publications.