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    products/sample-preparation-kits/hela-apoptosis-lysate-set-staurosporine-treated-and-vehicle-treated-control-ab136806.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Human HeLa
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HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control (ab136806)

  • Datasheet
  • SDS
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    Overview

    • Product name

      HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control
    • Product overview

      Staurosporine is a broad-spectrum inhibitor of serine/threonine kinases and tyrosine kinases. It is commonly used to induce apoptosis in experimental settings, although the mechanism is not fully understood.
      The staurosporine-treated HeLa lysate is designed for use as a western blot positive control for antibodies against apoptosis-specific proteins or for experimental treatments to induce apoptosis.

    • Notes

      Confluent HeLa cells were treated with either the vehicle (DMSO, 0.2%) or staurosporine (1 uM) for 4 hours. Cells were pelleted then lysed with Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM NaF, 1x Protease Inhibitors, 1% SDS) and the lysate cleared by sonication. The protein concentration was measured by BCA assay. Modified 5x SDS sample buffer was added to the lysate before storage at -80ºC. The lysates were validated by western blotting with the ab136812 Apoptosis WB cocktail (cleaved PARP, actin, caspase 3).

    • Tested applications

      Suitable for: WB, SDS-PAGEmore details

    Properties

    • Storage instructions

      Store at -80°C. Please refer to protocols.
    • Components 2 units
      Staurosporine-Treated HeLa Lysate 1 x 200µg
      Control for Staurosporine-Treated Lysate 1 x 200µg
    • Research areas

      • Kits/ Lysates/ Other
      • Lysates
      • Whole Cell Lysates
      • Human
      • HeLa

    Associated products

    • Related Products

      • Staurosporine, Protein kinase inhibitor (ab120056)
      • Apoptosis Western Blot Cocktail (pro/p17-caspase-3, cleaved PARP1, muscle actin) (ab136812)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab136806 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration.

    Suggested working concentration: 2.0 mg/mL, Load 20 µg/lane.

    User may choose to boil the sample before loading, at their discretion.

    SDS-PAGE
    Use at an assay dependent concentration.

    Suggested working concentration: 2.0 mg/mL, Load 20µg/lane.

    User may choose to boil the sample before loading, at their discretion.

    Notes
    WB
    Use at an assay dependent concentration.

    Suggested working concentration: 2.0 mg/mL, Load 20 µg/lane.

    User may choose to boil the sample before loading, at their discretion.

    SDS-PAGE
    Use at an assay dependent concentration.

    Suggested working concentration: 2.0 mg/mL, Load 20µg/lane.

    User may choose to boil the sample before loading, at their discretion.

    Images

    • Western Blot
      Western Blot
      Lane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 µM staurpsorine, 4 hours Load 20 µg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab136806? Please let us know so that we can cite the reference in this datasheet.

    ab136806 has not yet been referenced specifically in any publications.

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