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    products/sample-preparation-kits/hela-dna-damage-whole-cell-lysate-set-uv-treated-and-untreated-control-ab157396.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Human HeLa
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HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)

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Figure 1.  Western blot with ab136812 Apoptosis Western Blot Cocktail
  • Figure 2.  Western blot with ab133981 Anti-Caspase 9 antibody
  • Figure 3.  Western blot with ab131385 Apoptosis and DNA Damage Western Blot Cocktail

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Primary
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Anti-HA tag antibody - ChIP Grade (ab9110)

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Overview

  • Product name

    HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control
  • Species reactivity

    Reacts with: Human
  • Product overview

    UV light is a common source of DNA damage, and can lead to skin cancer and premature aging. Exposure to UV-B and UV-C light leads to the formation of pyrimidine dimers, and to a lesser extent, purine dimers and pyrimidine photophosphates. These dimerized DNA bases are typically removed by the nucleotide excision repair pathway. Failure to repair the damage can induce apoptosis by blocking DNA replication and transcription.


    The UV-treated HeLa lysate is designed for use as a western blot positive control when studying UV-induced DNA damage and/or apoptosis. Cells were treated with UV-C light for 1 minute, then cultured for 4 hours before collecting for lysates. Untreated cells were grown under standard cell culture conditions for HeLa cells.


    Samples are provided solubilized in an SDS-PAGE loading buffer, supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.


    Concentration: HeLa UV-treated lysate, 200 µg at 2.0 mg/mL
                                HeLa untreated lysate, 200 µg at 2.0 mg/mL

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 2 units
    Control for UV-Treated HeLa Lysate 1 x 200µg
    UV-Treated HeLa Lysate 1 x 200µg
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Human
    • HeLa

Associated products

  • Related Products

    • Apoptosis and DNA Damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western Blot Cocktail (ab131385)
    • Apoptosis Western Blot Cocktail (pro/p17-caspase-3, cleaved PARP1, muscle actin) (ab136812)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration.

Images

  • Figure 1.  Western blot with ab136812 Apoptosis Western Blot Cocktail
    Figure 1. Western blot with ab136812 Apoptosis Western Blot Cocktail
    Lane 1: MW marker
    Lane 2: Untreated HeLa lysate (ab157396)
    Lane 3: UV-treated HeLa lysate (ab157396).
    All lysates at 20 µg per lane.
    Primary antibodies (all lanes): ab136812 Apoptosis Western Blot Cocktail (pro/p17-caspase 3, cleaved-PARP, muscle actin) 1:250 dilution.
    Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution and Goat polyclonal to Rabbit IgG - HRP at 1:5000 dilution.
    Developed using the ECL method.
    Predicted band sizes: 89, 42, 32, 17 kDa
    Observed band sizes: 89, 42, 32 kDa
  • Figure 2.  Western blot with ab133981 Anti-Caspase 9 antibody
    Figure 2. Western blot with ab133981 Anti-Caspase 9 antibody
    Lane 1: MW marker
    Lane 2: Untreated HeLa lysate (ab157396)
    Lane 3: UV-treated HeLa lysate (ab157396)
    All lysates at 20 µg per lane.
    Primary antibody (all lanes): ab133981 Anti-Caspase 9 antibody at 2 µg/mL.
    Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.
    Developed using the ECL method.
    Predicted band size: 35 kDa
    Observed band size: 25 kDa
  • Figure 3.  Western blot with ab131385 Apoptosis and DNA Damage Western Blot Cocktail
    Figure 3. Western blot with ab131385 Apoptosis and DNA Damage Western Blot Cocktail
    Lanes 1, 4: MW marker
    Lane 2: Untreated HeLa lysate (ab157396)
    Lane 3: UV-treated HeLa lysate (ab157396).
    All lysates at 20 µg per lane.
    Primary antibodies:
    Lanes 1-3: ab131385 Apoptosis and DNA Damage Western Blot Cocktail (cleaved PARP, GAPDH, H2A.X pSer139) 1:250 dilution
    Lanes 4-6: H2A.X pSer139 antibody.
    Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.
    Developed using the ECL method.
    Predicted band sizes: 15, 36, 89 kDa
    Other bands observed (from H2A.X pSer139 antibody, identities unknown): 25, 30 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

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References (0)

Publishing research using ab157396? Please let us know so that we can cite the reference in this datasheet.

ab157396 has not yet been referenced specifically in any publications.

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