HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (ab135395)
Overview
-
Product name
HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated -
Product overview
HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.
Concentration:
HeLa Lysate - Vehicle Treated Control - 200 µg at 1 mg/mL
HeLa Lysate - Calyculin Treated - 200 µg at 1 mg/mL
The lysate is supplied in SDS-PAGE loading buffer with DTT and is ready to load on a gel.
-
Tested applications
Suitable for: WB, SDS-PAGEmore details
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 2 units Calyculin-Treated HeLa Lysate 1 x 200µg Control for Calyculin-Treated HeLa Lysate 1 x 200µg -
Research areas
Associated products
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration.
|
|
SDS-PAGE |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use at an assay dependent concentration. |
SDS-PAGE
Use at an assay dependent concentration. |
Images
-
All lanes: Anti-Phosphoserine/Threonine antibody (ab17464) at 1/1000
Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated Control at 20 µg
Secondary:
Goat polyclonal to Rabbit IgG – Pre-Adsorbed (HRP) at 1/6000 developed using the ECL technique.
Performed under reducing conditions. -
Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated control at 20 µg
Gel
10-20% acrylamide separation gel
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab135395 has not yet been referenced specifically in any publications.