HeLa Whole Cell RIPA Extract (ab170197)
Key features and details
- Sample type: Cell Lysate
Overview
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Product name
HeLa Whole Cell RIPA Extract -
Sample type
Cell Lysate -
Product overview
HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma. HeLa cells were lysed using radio immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors, centrifuged and the supernatant collected. Protein concentration of the extract was determined by BCA assay. This products is suitable for sandwich ELISAs, Immunoprecipitation, SDS-PAGE and Western Blotting.
Avoid freeze-thaw cycles with this product.
Concentration: Each unit contains a minimum of 300 µg at a lot-specific concentration. See label for concentration.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: SDS-PAGE, IP, WB, Blocking, Sandwich ELISAmore details
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 2 units HeLa Whole Cell RIPA Extract 2 vials -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| SDS-PAGE |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| Blocking |
Use at an assay dependent concentration.
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| Sandwich ELISA |
Use at an assay dependent concentration.
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| Notes |
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SDS-PAGE
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
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Blocking
Use at an assay dependent concentration. |
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Sandwich ELISA
Use at an assay dependent concentration. |
Images
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Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574) using HeLa Whole Cell RIPA Extract
Glucose 6 Phosphate Isomerase (G6PI) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574). A standard curve generated using the G6PI standard protein is shown (left image). G6PI signal using 2-fold serial dilutions of ab170197 starting at 50 µg/mL (right image). The G6PI concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 24.9 µg/mL.
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mtTFA (TFAM) Human ELISA Kit (ab123455) using HeLa Whole Cell RIPA ExtractMitochondrial Transcription Factor A (mtTFA) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using mtTFA (TFAM) Human ELISA Kit (ab123455). A standard curve generated using the mtTFA standard protein is shown (left panel). mtTFA signal using 2-fold serial dilutions of ab170197 starting at 400 µg/mL is shown (right panel). The mtTFA concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 841 ng/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170197 has not yet been referenced specifically in any publications.