Heparin Sepharose® (ab193268)
Key features and details
- Assay type: Bead-based sandwich immunoassay (quantitative, multiplexable)
- Sensitivity: 0.4 mg/ml
Overview
-
Product name
Heparin Sepharose® -
Assay type
Bead-based sandwich immunoassay (quantitative, multiplexable) -
Sensitivity
>= 0.4 mg/ml -
Product overview
High binding capacity (>0.4mg/mL). Minimal leaching of ligand. For column or batch purification of heparin-binding proteins & cation exchange (ab193268).
Contents:
Supplied as a 50% slurry in 20 % Ethanol; >2.5 mg heparin per mL Sepharose® beads.
Features:
Heparin beads have been widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, as well as DNA binding proteins (transcription factors, virus coat proteins etc). Abcam’s Heparin Sepharose® is designed for purification of heparin-binding proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. This Heparin Sepharose® formulation exhibits excellent binding capacity, high flow rate, no significant loss of the heparin ligand and a pH stability range of 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of heparin-binding proteins, enzymes or other ligands.
Sepharose is a registered trademark of GE Healthcare
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6553 Heparin Sepharose. 6553-10 is the same size as the 10 ml size of ab193268.
Heparin Sepharose® is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel.
Suggested Protocol:
- Wash column with ddH2O to remove air bubbles.
- Fill column with heparin beads.
- Wash the column with 5X volume of Binding Buffer.
- Dilute sample with Binding Buffer (1:1 ratio) or change the sample solution to binding buffer by means of your choice.
- Add the sample solution onto the column.
- Collect the solution and repeat step 5 & 6 several times if necessary.
- Wash the column 5-10 times with the Binding Buffer.
- Add Elution Buffer to elute bound protein.
- Collect the eluent using microcentrifuge tube.
- Assay protein concentration and combine the fractions containing sufficient heparin- binding protein.
- Bead can be cleaned and regenerated by washing with 2-3x volume of high concentration salt solution and then the binding buffer.
-
Tested applications
Suitable for: Purificationmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 10 ml 50 ml Heparin Sepharose® 1 x 1ml 1 x 10ml 1 x 50ml -
Research areas
Associated products
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Purification |
Use at an assay dependent concentration.
Purification of heparin-binding proteins, enzymes or other ligands. |
| Notes |
|---|
|
Purification
Use at an assay dependent concentration. Purification of heparin-binding proteins, enzymes or other ligands. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab193268 has not yet been referenced specifically in any publications.