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Immunoprecipitation kit (ab206996)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (45)

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Immunoprecipitation - Immunoprecipitation kit (ab206996)
  • Functional Studies - Immunoprecipitation kit (ab206996)
  • Comparison of Binding Capacity of Protein-A and Protein-A/G Beads.

Key features and details

  • Sample type: Adherent cells, Suspension cells, Tissue

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Overview

  • Product name

    Immunoprecipitation kit
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Product overview

    Abcam’s Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.


    Abcam’s Immunoprecipitation Kit provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps. The Protein A/G Sepharose® beads provided in the kit have a higher binding capacity with broader antibody isotype binding than traditional Protein A or Protein G resins.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K286 Immunoprecipitation (IP) Kit. K286-25 is the same size as the 25 test size of ab206996.

  • Tested applications

    Suitable for: IPmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 25 tests
    10X Wash Buffer 1 x 20ml
    Lysis Buffer (non-denaturing) 1 x 40ml
    Protease Inhibitor Cocktail (lyophilized) 1 vial
    Protein A/G Sepharose® 1 x 1ml
    RIPA Lysis Buffer 1 x 40ml

Associated products

  • Related Products

    • ChIP Kit - One Step (ab117138)
    • High-Sensitivity ChIP Kit (ab185913)
    • ChIP Kit (ab500)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
Notes
IP
Use at an assay dependent concentration.

Images

  • Immunoprecipitation - Immunoprecipitation kit (ab206996)
    Immunoprecipitation - Immunoprecipitation kit (ab206996)Image from House et al., BMC Cancer, 18(1):595; doi: 10.1186/s12885-018-4507-2. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Co-immunoprecipitation experiments indicate IKKε does not interact with NIK or IKKε.Immunoprecipitation was performed on MDA MB 468 cells using the Abcam immunoprecipitation kit (ab206996), according to manufacturer's instructions. Briefly, non-denaturing lysis buffer was used to collect 300 µg of cell lysate was incubated overnight with 3 µg/ml of either control rabbit IgG or IKKε rabbit polyclonal antibody (ab7891). Antibody bound proteins were captured using protein A/G sepharose beads, eluted, and analyzed via western blot.

  • Functional Studies - Immunoprecipitation kit (ab206996)
    Functional Studies - Immunoprecipitation kit (ab206996)Lunova, Mariia et al., Scientific reportsvol. 7,1 16049., Fig 8, doi:10.1038/s41598-017-16447-6

    Huh7 cultured with Si-OH, Si-NH2, or PS-NH2 NPs (all 50 µg/ml) bearing BSA or RNase (both 50 µM) as hard protein corona or bare NPs for 4 h. After 4 h post NP treatment, cells were lysed with lysis buffer from immunoprecipitation kit (Abcam). RagC-mTOR complexes were co-immunoprecipitated from the precleared cell lysates with appropriate Ab as described in the manufacturer's instruction. The resulting protein complex was eluted from the beads with Laemmli protein sample buffer for SDS-PAGE and resolved on SDS-PAGE with specific antibody against mTOR

  • Comparison of Binding Capacity of Protein-A and Protein-A/G Beads.
    Comparison of Binding Capacity of Protein-A and Protein-A/G Beads.

    Comparison of immunoprecipitation using Protein A beads and Immunoprecipitation kit ab206996. HDAC2 activity assay of the antigen-antibody complex captured using Protein A beads or ab206996 by following the same protocol demonstrates that ab206996 is more efficient in IP than Protein A beads.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (45)

Publishing research using ab206996? Please let us know so that we can cite the reference in this datasheet.

ab206996 has been referenced in 45 publications.

  • Mehta SL  et al. Tau and GSK-3β are Critical Contributors to α-Synuclein-Mediated Post-Stroke Brain Damage. Neuromolecular Med 25:94-101 (2023). PubMed: 36447045
  • Kammala A  et al. Functional role and regulation of permeability-glycoprotein (P-gp) in the fetal membrane during drug transportation. Am J Reprod Immunol 87:e13515 (2022). PubMed: 34873775
  • Manzoor S  et al. MLH1 mediates cytoprotective nucleophagy to resist 5-Fluorouracil-induced cell death in colorectal carcinoma. Neoplasia 24:76-85 (2022). PubMed: 34952246
  • Zhang X  et al. CPNE3 interaction with RACK1 protects against myocardial ischemia/reperfusion injury. Exp Ther Med 23:128 (2022). PubMed: 34970351
  • D'Onofrio N  et al. Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway. FEBS Lett 596:1313-1329 (2022). PubMed: 35122251
View all Publications for this product

Customer reviews and Q&As

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Question

a customer of ours would like to get some information about ab206996.

In particular she would like to have confirmation tha Protease Inhibitor Cocktail (lyophilized) doesn’t contain phosphatase inhibitor.

If it doesn’t contain, is it possible to add phosphatase inhibitors by my customer?

Thanks and kind regards

Read More

Abcam community

Verified customer

Asked on Jun 13 2017

Answer

I can confirm that the protease inhibitor cocktail provided with ab206996 Immunoprecipitation kit does not contain phosphatase inhibitors.

Read More

Abcam Scientific Support

Answered on Jun 13 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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