Immunoprecipitation kit (ab206996)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Immunoprecipitation kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
Abcam’s Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.
Abcam’s Immunoprecipitation Kit provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps. The Protein A/G Sepharose® beads provided in the kit have a higher binding capacity with broader antibody isotype binding than traditional Protein A or Protein G resins.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K286 Immunoprecipitation (IP) Kit. K286-25 is the same size as the 25 test size of ab206996.
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Tested applications
Suitable for: IPmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests 10X Wash Buffer 1 x 20ml Lysis Buffer (non-denaturing) 1 x 40ml Protease Inhibitor Cocktail (lyophilized) 1 vial Protein A/G Sepharose® 1 x 1ml RIPA Lysis Buffer 1 x 40ml
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
Images
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Immunoprecipitation - Immunoprecipitation kit (ab206996)Image from House et al., BMC Cancer, 18(1):595; doi: 10.1186/s12885-018-4507-2. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Co-immunoprecipitation experiments indicate IKKε does not interact with NIK or IKKε.Immunoprecipitation was performed on MDA MB 468 cells using the Abcam immunoprecipitation kit (ab206996), according to manufacturer's instructions. Briefly, non-denaturing lysis buffer was used to collect 300 µg of cell lysate was incubated overnight with 3 µg/ml of either control rabbit IgG or IKKε rabbit polyclonal antibody (ab7891). Antibody bound proteins were captured using protein A/G sepharose beads, eluted, and analyzed via western blot.
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Functional Studies - Immunoprecipitation kit (ab206996)Lunova, Mariia et al., Scientific reportsvol. 7,1 16049., Fig 8, doi:10.1038/s41598-017-16447-6
Huh7 cultured with Si-OH, Si-NH2, or PS-NH2 NPs (all 50 µg/ml) bearing BSA or RNase (both 50 µM) as hard protein corona or bare NPs for 4 h. After 4 h post NP treatment, cells were lysed with lysis buffer from immunoprecipitation kit (Abcam). RagC-mTOR complexes were co-immunoprecipitated from the precleared cell lysates with appropriate Ab as described in the manufacturer's instruction. The resulting protein complex was eluted from the beads with Laemmli protein sample buffer for SDS-PAGE and resolved on SDS-PAGE with specific antibody against mTOR
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Comparison of immunoprecipitation using Protein A beads and Immunoprecipitation kit ab206996. HDAC2 activity assay of the antigen-antibody complex captured using Protein A beads or ab206996 by following the same protocol demonstrates that ab206996 is more efficient in IP than Protein A beads.
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab206996 has been referenced in 45 publications.
- Mehta SL et al. Tau and GSK-3β are Critical Contributors to α-Synuclein-Mediated Post-Stroke Brain Damage. Neuromolecular Med 25:94-101 (2023). PubMed: 36447045
- Kammala A et al. Functional role and regulation of permeability-glycoprotein (P-gp) in the fetal membrane during drug transportation. Am J Reprod Immunol 87:e13515 (2022). PubMed: 34873775
- Manzoor S et al. MLH1 mediates cytoprotective nucleophagy to resist 5-Fluorouracil-induced cell death in colorectal carcinoma. Neoplasia 24:76-85 (2022). PubMed: 34952246
- Zhang X et al. CPNE3 interaction with RACK1 protects against myocardial ischemia/reperfusion injury. Exp Ther Med 23:128 (2022). PubMed: 34970351
- D'Onofrio N et al. Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway. FEBS Lett 596:1313-1329 (2022). PubMed: 35122251