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    products/sample-preparation-kits/nuclear-extraction-kit-ab221978.pdf

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Nuclear Extraction Kit (ab221978)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (3)

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Key features and details

  • Sample type: Adherent cells, Suspension cells, Tissue

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Overview

  • Product name

    Nuclear Extraction Kit
    See all Nuclear Extraction kits
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Product overview

    This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.  


     

  • Notes

     

     

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 100 tests
    Nonidet P-40 Assay Reagent (10%) 1 x 6ml
    Nuclear Extraction Buffer (2X) 1 x 3ml
    Nuclear Extraction Dithiothreitol (1M) 1 x 60µl
    Nuclear Extraction Hypotonic Buffer (10X) 1 x 6ml
    Nuclear Extraction PBS (10X) 1 x 100ml
    Nuclear Extraction Phosphatase Inhibitors (50X) 2 x 5ml
    Nuclear Extraction Protease Inhibitor Cocktail (100X) 1 x 650µl

Associated products

  • Related Products

    • BCA Protein Assay Kit (ab102536)

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab221978? Please let us know so that we can cite the reference in this datasheet.

ab221978 has been referenced in 3 publications.

  • Daoud F  et al. YAP and TAZ in Vascular Smooth Muscle Confer Protection Against Hypertensive Vasculopathy. Arterioscler Thromb Vasc Biol 42:428-443 (2022). PubMed: 35196875
  • Zheng LZ  et al. Magnesium and vitamin C supplementation attenuates steroid-associated osteonecrosis in a rat model. Biomaterials 238:119828 (2020). PubMed: 32045781
  • Radic M  et al. The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage. Int J Mol Sci 21:N/A (2020). PubMed: 32235358

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