Protein A Agarose (High Affinity) (ab193255)
Key features and details
- Sample type: Ascites Fluid, Cell culture media, Cell culture supernatant, Serum
Overview
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Product name
Protein A Agarose (High Affinity) -
Sample type
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
Product overview
Very high binding capacity (>35 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation & ChIP (ab193255).
Contents:
Supplied as 50% slurry in 20% Ethanol.
Features:
High binding capacity = Binding of IgG ≥ 36 mg human or rabbit IgG/mL Protein A Agarose.
Minimal leaching of the ligand
Flow Rate Tested* = 2.89 mL/min.
*Test conditions: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Maximum Flow Rate** = 1800 cm/hr; minimum leaching of recombinant Protein A.
**NOTE = the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.
Usage = Reusable for up to 10 times without significant loss of binding capacity.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6520 Hi-Bind™ Protein A-Agarose. 6520-100 is the same size as the 100 ml size of ab193255.
Abcam's Protein A Agarose (High Affinity) beads are specially prepared for high IgG binding by covalently coupling recombinant Protein A to 6% cross-linked agarose beads, the most popular resin for protein affinity purification methods. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A compared to standard Protein A agarose beads. The IgG binding capacity of Protein A Agarose (High Affinity) is ≥ 36 mg human or rabbit IgG per mL of wet beads. The Protein A Agarose (High Affinity) beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
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Tested applications
Suitable for: IP, Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml 25 ml 100 ml Protein A-Agarose (High Affinity) 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
|
| Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
|
Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab193255 has been referenced in 3 publications.
- Zhang L et al. S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis. Cell Mol Gastroenterol Hepatol 11:697-724 (2021). PubMed: 33075563
- Wang Y et al. CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA. BMC Biol 18:115 (2020). PubMed: 32887607
- Wu J et al. PHA-4/FoxA senses nucleolar stress to regulate lipid accumulation in Caenorhabditis elegans. Nat Commun 9:1195 (2018). PubMed: 29567958