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  1. Link

    products/sample-preparation-kits/protein-ag-sepharose-ab193262.pdf

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Kits/ Lysates/ Other Kits Molecular Biology Kits Beads Protein A/G
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Protein A/G Sepharose® (ab193262)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (8)

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Overview

  • Product name

    Protein A/G Sepharose®
  • Product overview

    High binding capacity Protein A/G Sepharose (>20 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG.


    Contents:


    Supplied as a 50% slurry in 20% Ethanol; >1.5 mg Protein A/G per mL Sepharose® beads.


    Features:


    Binding capacity greater than 20 mg of wet gel; high flow rate; low falling off of rProtein A/G; pH stability 2-10.


    These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.


    Store beads at 4°C.


    The beads may be damaged above 40°C.


    DO NOT FREEZE.


    Wash beads 3 times with 3x bead volume of desired buffer before use.


     


    Applications:


    Purification of monoclonal and polyclonal antibodies.


     


    Sepharose is a registered trademark of GE Healthcare

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called 6503 Protein A/G Sepharose. 6503-1 is the same size as the 1 ml size of ab193262.

    Protein A/G Sepharose® is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G) to 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 20 mg of rabbit IgG per ml of wet gel.

  • Tested applications

    Suitable for: Purificationmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 ml 5 ml 25 ml 100 ml
    Protein A/G Sepharose® 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Molecular Biology Kits
    • Beads
    • Protein A/G

Associated products

  • Related Products

    • Bradford Reagent (ab119216)
    • Optiblot SDS-PAGE Sample Preparation Kit (ab133414)
    • Cell Lysis Buffer (ab152163)
    • 10X RIPA Buffer (ab156034)
    • Native lysis Buffer (ab156035)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification
Use at an assay dependent concentration.

Purification of monoclonal and polyclonal antibodies.

Notes
Purification
Use at an assay dependent concentration.

Purification of monoclonal and polyclonal antibodies.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab193262? Please let us know so that we can cite the reference in this datasheet.

ab193262 has been referenced in 8 publications.

  • Zhao Z  et al. Dexmedetomidine inhibits the PSD95-NMDA receptor interaction to promote functional recovery following traumatic brain injury. Exp Ther Med 21:4 (2021). PubMed: 33235613
  • Tao Z  et al. Sirt1 coordinates with ERa to regulate autophagy and adiposity. Cell Death Discov 7:53 (2021). PubMed: 33723227
  • Kovács RÁ  et al. Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice. Front Immunol 11:599771 (2020). PubMed: 33628204
  • Wei M  et al. NDRG2 regulates adherens junction integrity to restrict colitis and tumourigenesis. EBioMedicine 61:103068 (2020). PubMed: 33099085
  • Le-Bel G  et al. Analysis of the proteasome activity and the turnover of the serotonin receptor 2B (HTR2B) in human uveal melanoma. Exp Eye Res 184:72-77 (2019). PubMed: 31002821
  • Song H  et al. METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes. Autophagy 15:1419-1437 (2019). PubMed: 30870073
  • Gao Y  et al. Effects of farnesoid-X-receptor SUMOylation mutation on myocardial ischemia/reperfusion injury in mice. Exp Cell Res 371:301-310 (2018). PubMed: 30098335
  • Albrecht LV  et al. Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking. Proc Natl Acad Sci U S A 115:E5317-E5325 (2018). PubMed: 29773710

Customer reviews and Q&As

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great for academic lab

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
great
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jul 11 2022

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