Protein A/G Sepharose® Column (ab193263)
Overview
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Product name
Protein A/G Sepharose® Column -
Product overview
Contents:
Supplied as ready-to-use pre-packed columns of 1 mL or 5 mL bead volume in PBS with 0.02% sodium azide.
Features:
Binding capacity of human IgG greater than 10 mg/mL of bead; high flow rate; low falling off of rProtein A/G; pH stability 2-10.
Store column at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of monoclonal and polyclonal antibodies.
Sepharose is a registered trademark of GE Healthcare
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6528 Protein A/G Sepharose Column. 6528-5 is the same size as the 5 ml size of ab193263.
Protein A/G Sepharose® is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G) to 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet bead.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml Protein A/G Sepharose® Column 1 x 1ml 1 x 5ml -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies. |
| Notes |
|---|
|
Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab193263 has not yet been referenced specifically in any publications.