Whole Cell Extraction Kit (ab113475)
Key features and details
- Assay time: 45 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Whole Cell Extraction Kit -
Sample type
Adherent cells, Suspension cells -
Assay time
0h 45m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
Abcam's Whole Cell Extraction Kit (ab113475) provides a simple and selective method for extracting whole cell proteins in just 45 minutes for a variety of applications such as western blotting, cellular enzyme assays, or any other procedures requiring cellular proteins. ab113475 can be used to extract cellular proteins from mammalian cells and tissue samples.
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Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests 1000X DTT Solution 1 x 100µl 1000X Protease Inhibitor Cocktail 1 x 100µl 5X Extraction Buffer 1 x 20ml -
Research areas
Images
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Functional Studies - Whole Cell Extraction Kit (ab113475)Whole cell extracts were prepared from MCF-7 cells using ab113475. Activity of SOD was measured using different amounts of the extract.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab113475 has been referenced in 4 publications.
- Al-Dwairi A et al. Metformin exerts anti-inflammatory effects on mouse colon smooth muscle cells in vitro. Exp Ther Med 16:985-992 (2018). PubMed: 30116349
- Hannibal L et al. Transcellular transport of cobalamin in aortic endothelial cells. FASEB J 32:5506-5519 (2018). PubMed: 29741927
- Zhu B et al. Rapid identification of ubiquitination and SUMOylation target sites by microfluidic peptide array. Biochem Biophys Rep 5:430-438 (2016). PubMed: 27047992
- Peng YT et al. Upregulation of cyclin-dependent kinase inhibitors CDKN1B and CDKN1C in hepatocellular carcinoma-derived cells via goniothalamin-mediated protein stabilization and epigenetic modifications. Toxicol Rep 2:322-332 (2015). PubMed: 28962365