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    products/sample-preparation-kits/whole-cell-extraction-kit-ab113475.pdf

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Whole Cell Extraction Kit (ab113475)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (4)

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Functional Studies - Whole Cell Extraction Kit (ab113475)

    Key features and details

    • Assay time: 45 min
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      Whole Cell Extraction Kit
    • Sample type

      Adherent cells, Suspension cells
    • Assay time

      0h 45m
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Mammals
    • Product overview

      Abcam's Whole Cell Extraction Kit (ab113475) provides a simple and selective method for extracting whole cell proteins in just 45 minutes for a variety of applications such as western blotting, cellular enzyme assays, or any other procedures requiring cellular proteins. ab113475 can be used to extract cellular proteins from mammalian cells and tissue samples.

      Not sure if this is the right product for you? Check out our Sample Preparation Guide for help.

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 100 tests
      1000X DTT Solution 1 x 100µl
      1000X Protease Inhibitor Cocktail 1 x 100µl
      5X Extraction Buffer 1 x 20ml
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • Sample Preparation
      • Epigenetics and Nuclear Signaling
      • Assays & Kits
      • Sample preparation

    Images

    • Functional Studies - Whole Cell Extraction Kit (ab113475)
      Functional Studies - Whole Cell Extraction Kit (ab113475)
      Whole cell extracts were prepared from MCF-7 cells using ab113475. Activity of SOD was measured using different amounts of the extract.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab113475? Please let us know so that we can cite the reference in this datasheet.

    ab113475 has been referenced in 4 publications.

    • Al-Dwairi A  et al. Metformin exerts anti-inflammatory effects on mouse colon smooth muscle cells in vitro. Exp Ther Med 16:985-992 (2018). PubMed: 30116349
    • Hannibal L  et al. Transcellular transport of cobalamin in aortic endothelial cells. FASEB J 32:5506-5519 (2018). PubMed: 29741927
    • Zhu B  et al. Rapid identification of ubiquitination and SUMOylation target sites by microfluidic peptide array. Biochem Biophys Rep 5:430-438 (2016). PubMed: 27047992
    • Peng YT  et al. Upregulation of cyclin-dependent kinase inhibitors CDKN1B and CDKN1C in hepatocellular carcinoma-derived cells via goniothalamin-mediated protein stabilization and epigenetic modifications. Toxicol Rep 2:322-332 (2015). PubMed: 28962365

    Customer reviews and Q&As

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    Question

    Dear All,
    our customer is interested in your ab113475 but I saw that the lead time on this item is currently 1-2 weeks.
    Could you please confirm that or is it available?

    Thanks in advance
    Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on May 10 2012

    Answer

    Thank you for your inquiry.

    I am sorry to confirm that the information on the datasheet is correct.

    This product has currently a lead time of 1-2 weeks and is not in stock at the moment.

    Read More

    Abcam Scientific Support

    Answered on May 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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