Donkey F(ab) Anti-Goat IgG H&L (HRP) (ab6667)
Key features and details
- Donkey F(ab) Anti-Goat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, Dot blot, ELISA, Electron Microscopy, IHC, ICC/IF
Overview
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Product name
Donkey F(ab) Anti-Goat IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c). -
Tested applications
Suitable for: WB, Dot blot, ELISA, Electron Microscopy, IHC, ICC/IFmore details -
Immunogen
Goat IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000.
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| Dot blot |
Use at an assay dependent dilution.
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| ELISA |
1/17000. This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
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| Electron Microscopy |
Use at an assay dependent dilution.
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| IHC |
Use at an assay dependent dilution.
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| ICC/IF |
1/500 - 1/2500.
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| Notes |
|---|
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WB
1/2000 - 1/10000. |
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Dot blot
Use at an assay dependent dilution. |
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ELISA
1/17000. This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. |
|
Electron Microscopy
Use at an assay dependent dilution. |
|
IHC
Use at an assay dependent dilution. |
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ICC/IF
1/500 - 1/2500. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab6667 has been referenced in 5 publications.
- Sakamoto Y et al. High-Fat Diet and Age-Dependent Effects of IgA-Bearing Cell Populations in the Small Intestinal Lamina Propria in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33503874
- Jigjidkhorloo N et al. Expression of L-type amino acid transporter 1 is a poor prognostic factor for Non-Hodgkin's lymphoma. Sci Rep 11:21638 (2021). PubMed: 34737339
- Marsala M et al. Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats. Stem Cells Transl Med 9:177-188 (2020). PubMed: 31800978
- Li BY et al. High expression of RAB38 promotes malignant progression of pancreatic cancer. Mol Med Rep 19:909-918 (2019). PubMed: 30569114
- Büttner-Mainik A et al. Production of biologically active recombinant human factor H in Physcomitrella. Plant Biotechnol J 9:373-83 (2011). PubMed: 20723134
