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Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)

Submit a review Q&A (4)References (2)

Key features and details

  • Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt

Overview

  • Product name

    Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Human

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details

  • Immunogen

    Human IgG whole molecule

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.5
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

  • Conjugation notes

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8

  • Clonality

    Polyclonal

  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.
Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab7005? Please let us know so that we can cite the reference in this datasheet.

ab7005 has been referenced in 2 publications.

  • Avivi-Mintz S  et al. Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System. Biosensors (Basel) 12:N/A (2021). PubMed: 35049635
  • Zhang Y  et al. Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes. Int J Mol Med 41:77-86 (2018). PubMed: 29115390

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

We have a customer who is experiencing troubles with your Human IgG antibody (ab7005). Please refer to below: ?We have purchased this antibody on 2 occasions now and have had no success in detecting PE fluorescence by flow cytometry in our IgG expressing cells. I note that the Immunogen is "Human IgG whole molecule" and I would like some clarification whether this means the antibody specifically recognizes the Fab portion (or hypervariable) portion of our IgG immunoglobulin alone. I also note that the specification sheet denotes under Relevance ".. absence of F(c) mediated binding" and therefore assume that this means that the antibody does not recognise the Fc portion of our secreted IgG immunoglobulin. Essentially, I am trying to detect a membrane bound IgG immunoglobulin, however I am not sure which domain (Fc or Fab) of our IgG molecule are exposed to the extracellular environment and as such whether using ab7005 is preferential to the hypervariable /Fab region only Clearly, if this is the case then we would need antibody which recognises the Fc portion in order to test our hypothesis.?. If you require any further information please do not hesitate to contact me.

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Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

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Question

We have a customer who is experiencing troubles with your Human IgG antibody (ab7005). Please refer to below: ?We have purchased this antibody on 2 occasions now and have had no success in detecting PE fluorescence by flow cytometry in our IgG expressing cells. I note that the Immunogen is "Human IgG whole molecule" and I would like some clarification whether this means the antibody specifically recognizes the Fab portion (or hypervariable) portion of our IgG immunoglobulin alone. I also note that the specification sheet denotes under Relevance ".. absence of F(c) mediated binding" and therefore assume that this means that the antibody does not recognise the Fc portion of our secreted IgG immunoglobulin. Essentially, I am trying to detect a membrane bound IgG immunoglobulin, however I am not sure which domain (Fc or Fab) of our IgG molecule are exposed to the extracellular environment and as such whether using ab7005 is preferential to the hypervariable /Fab region only Clearly, if this is the case then we would need antibody which recognises the Fc portion in order to test our hypothesis.?. If you require any further information please do not hesitate to contact me.

Read More
Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

Read More

Question

How many micrograms are in the anti-IgG ab 7005....it gives microlitres but not micrograms....thanks

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Answer

Thank you for your enquiry. The concentration of this product is 0.5 mg/mL. The total would be 500ug.

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Question

Customer would like to know the Phycobiliprotein Concentration of ab7005, and what is the ratio of antibody to PE for ab7005 and ab7006.

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Answer

The Phycobiliprotein concentration of ab7005 is 0.5mg/mL. For both ab7005 and ab7006, there is one PE molecule with at least one molecule of active antibody. If you have any more questions, please contact us again.

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