Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
Key features and details
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed
- Conjugation: Alexa Fluor® 750. Ex: 749nm, Em: 775nm
- Host species: Donkey
- Suitable for: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-P
Related conjugates and formulations
Overview
-
Product name
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-Pmore details -
Minimal
cross-reactivity
Chicken, Human, Mouse, Pig, Rabbit, Rat more details -
Immunogen
Full length protein corresponding to Goat IgG.
-
Conjugation
Alexa Fluor® 750. Ex: 749nm, Em: 775nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
-
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
This applicaiton is no longer batch tested |
|
| ELISA |
Use at an assay dependent concentration.
This applicaiton is no longer batch tested |
|
| ICC/IF |
1/200 - 1/1000.
This applicaiton is no longer batch tested |
|
| Flow Cyt |
1/2000.
This applicaiton is no longer batch tested |
|
| IHC-P |
Use at an assay dependent concentration.
This applicaiton is no longer batch tested |
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. This applicaiton is no longer batch tested |
|
ELISA
Use at an assay dependent concentration. This applicaiton is no longer batch tested |
|
ICC/IF
1/200 - 1/1000. This applicaiton is no longer batch tested |
|
Flow Cyt
1/2000. This applicaiton is no longer batch tested |
|
IHC-P
Use at an assay dependent concentration. This applicaiton is no longer batch tested |
Images
-
Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)Anti-GAPDH antibody - Loading Control (ab9483) at 1 µg/ml + HeLa Whole Cell Lysate at 10 µg
Secondary
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 37 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab9483 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
-
Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml + HeLa Whole Cell Lysate at 10 µg
Secondary
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 42 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
-
Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab175745 has been referenced in 1 publication.
- Etxeberria-Rekalde E et al. Quantification of Huntington's Disease Related Markers in the R6/2 Mouse Model. Front Mol Neurosci 13:617229 (2020). PubMed: 33505246