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  1. Link

    products/secondary-antibodies/donkey-goat-igg-hl-alexa-fluor-750-preadsorbed-ab175745.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
  • Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)

Key features and details

  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed
  • Conjugation: Alexa Fluor® 750. Ex: 749nm, Em: 775nm
  • Host species: Donkey
  • Suitable for: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-P

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-Pmore details
  • Minimal
    cross-reactivity


    Chicken, Human, Mouse, Pig, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation

    Alexa Fluor® 750. Ex: 749nm, Em: 775nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • Alexa Fluor® 750

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.

This applicaiton is no longer batch tested

ELISA
Use at an assay dependent concentration.

This applicaiton is no longer batch tested

ICC/IF
1/200 - 1/1000.

This applicaiton is no longer batch tested

Flow Cyt
1/2000.

This applicaiton is no longer batch tested

IHC-P
Use at an assay dependent concentration.

This applicaiton is no longer batch tested

Notes
IHC-Fr
Use at an assay dependent concentration.

This applicaiton is no longer batch tested

ELISA
Use at an assay dependent concentration.

This applicaiton is no longer batch tested

ICC/IF
1/200 - 1/1000.

This applicaiton is no longer batch tested

Flow Cyt
1/2000.

This applicaiton is no longer batch tested

IHC-P
Use at an assay dependent concentration.

This applicaiton is no longer batch tested

Images

  • Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
    Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
    Anti-GAPDH antibody - Loading Control (ab9483) at 1 µg/ml + HeLa Whole Cell Lysate at 10 µg

    Secondary
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 37 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab9483 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
    Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
    Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml + HeLa Whole Cell Lysate at 10 µg

    Secondary
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 42 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
    Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab175745? Please let us know so that we can cite the reference in this datasheet.

ab175745 has been referenced in 1 publication.

  • Etxeberria-Rekalde E  et al. Quantification of Huntington's Disease Related Markers in the R6/2 Mouse Model. Front Mol Neurosci 13:617229 (2020). PubMed: 33505246

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