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Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6886)

Submit a review Q&A (4)References (2)
Western blot - Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6886)

    Key features and details

    • Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)
    • Conjugation: Alkaline Phosphatase
    • Host species: Donkey
    • Suitable for: Dot blot, ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

    Overview

    • Product name

      Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)
      See all IgG secondary antibodies

    • Host species

      Donkey

    • Target species

      Goat

    • Tested applications

      Suitable for: Dot blot, ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

    • Immunogen

      Full length native Goat IgG (purified).

    • Conjugation

      Alkaline Phosphatase

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.

    • Conjugation notes

      Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

    • Clonality

      Polyclonal

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6886 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Dot blot
    Use at an assay dependent dilution.
    ICC/IF
    1/200 - 1/1000.
    ELISA
    1/8300.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/500 - 1/2500.
    Notes
    Dot blot
    Use at an assay dependent dilution.
    ICC/IF
    1/200 - 1/1000.
    ELISA
    1/8300.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/500 - 1/2500.

    Images

    • Western blot - Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6886)
      Western blot - Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6886)

      ab6886 was used at dilution 1/2500 with the primary antibody ab7613 in WB. See the review on ab7613.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab6886? Please let us know so that we can cite the reference in this datasheet.

    ab6886 has been referenced in 2 publications.

    • Skrzypek K  et al. A High Cell-Bearing Capacity Multibore Hollow Fiber Device for Macroencapsulation of Islets of Langerhans. Macromol Biosci 20:e2000021 (2020). PubMed: 32567161
    • Rudnik-Jansen I  et al. Applicability of a Modified Rat Model of Acute Arthritis for Long-Term Testing of Drug Delivery Systems. Pharmaceutics 11:N/A (2019). PubMed: 30736430

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    I performed Western blot with primary antibody (ab9132) and secondary antibody (ab6886). But no signal was detected. Is the secondary antibody correct in use ?

    Read More
    Answer

    The secondary antibody ab6886 will recognize the anti-Myc tag antibody (ab9132) which you are using. This secondary is Alkaline Phosphatase conjugated, in order to detect this in Western Blot you will need to use a substrate which will react with AP. These are generally chromogenic substrates such as NBT/BCIP. Abcam do not recommend chromogenic kits suc as BCIP/NBT detection kits for Western Blots as they are not as sensitive as enhanced ECL or fluorescent methods however that are used successfully by a number of researchers visualizing high expressed proteins.

    Read More

    Question

    Please, Could you send me compliance certificate

    AB6908 GR55986
    AB6900 GR54072
    AB6886 GR14049
    AB103742 GR66527
    AB7413 GR64347

    Read More
    Answer

    Thank you for contacting us.

    As requested, I have attached the Certificates of Compliance for the antibodies and reagents you mentioned - to this email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    ich bin an einer immunohistochemischen Färbung von humanem CD8 und humanem FoxP3 (in Mausgewebe) interessiert.



    - Idealerweise mit einen rabbit anti CD-8 Primärantikörper

    - Und FoxP3 mit Alkalischer Phosphatase (o.a.) gelabeltem Sekundärantikörper, um ggf. später ein doublestaining zu machen



    Dazu hätte ich zwei Fragen: Welcher Unterschied besteht zwischen Antikörpern aus Überstanden bzw. aufgereinigten Antikörpern? Gibt es spezielle Vor-oder Nachteile bei den jeweiligen Methoden?



    Würden Sie mir bitte ein Angebot mit jeweils passenden Sekundärantikörper machen?

    Read More
    Answer

    Vielen Dank für Ihre Anfrage.

    Ich habe Ihnen hier vier Antikörper herausgesucht, die wenig bzw. gar keine Kreuzreaktion mit dem Maus-Protein aufweisen, so dass Sie nur das humane Protein im Mausgewebe detektieren sollten.

    Drei der vier Antikörper stammen aus dem Kaninchen, falls Sie also eine Doppelfärbung durchführen möchten, wurde ich Vorschlagen, einen der Rabbit Antikörper mit einem ungelabelten Sekundär- Antikörper zu blockieren, und dann den sekundären Antikörper mit einem tertiären Antikörper nachzuweisen: zb. im Falle der Kombination aus ab93278 und ab10563.

    Dazu passen wuerden dann die folgenden Antikörper: ab6831 Chicken polyclonal Secondary Antibody to Rabbit IgG - H&L gefolgt von ab97142 Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alkaline Phosphatase), pre-adsorbed ; und ab7084 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed.

    Oder aber Sie benutzen zwei primäre Antikörper aus zwei verschiedenen Species (ab2481 und ab4055).

    Als Sekundär- Antikoerper kommen dann ab7084 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed; und ab6886 Donkey anti-Goat IgG H&L (AP) secondary antibody.

    Bezüglich Ihrer Frage nach dem Aufreinigungsgrad: Der einzige Vorteil eines aufgereinigten Antikörpers ist es, dass Sie eine Isotopenkontrolle berechnen können, da Sie genau wissen, wie viel Antikörper Sie einsetzen. Dies ist aber eigentlich nur in der Flow Zytometrie von Interesse.


    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
    https://www.abcam.com/abreviews

    Read More

    Question

    Could you suggest anti ab9871 secondary antibodies that can be sued in ELISA and ICC/IF?

    Read More
    Answer

    Thank you for contacting us.

    As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

    Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However these will not work in Immunofluorescence unless further steps in protocol applied.

    We have FITC, TRITC or Cy5 conjugated antibodies in catalogue that can work in Immunofluorescence and Fluorescence ELISA.

    Another option is choosing Biotin conjugated secondary antibody and then buying strepavidin or streptavidin conjugated HRP (ab7403).

    ab5753, ab6740, ab6884, ab7124, ab7131 coul dbe a good option.

    I know it sounds complicated however this is only way to combine ELISA and ICC/IF with one secondary antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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