Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97107)
Key features and details
- Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
|
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| IHC-P |
1/100 - 1/1000.
|
|
| ELISA |
1/5000 - 1/50000. (Primary).
|
|
| WB |
1/5000 - 1/50000. (Chemiluminescent).
|
| Notes |
|---|
|
ICC
Use at an assay dependent dilution. |
|
IHC-P
1/100 - 1/1000. |
|
ELISA
1/5000 - 1/50000. (Primary). |
|
WB
1/5000 - 1/50000. (Chemiluminescent). |
Images
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Western blot - Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97107)All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.
Secondary
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/5000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/20000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 8 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab97107 has been referenced in 3 publications.
- Edwards MJ et al. Sphingosine prevents binding of SARS-CoV-2 spike to its cellular receptor ACE2. J Biol Chem 295:15174-15182 (2020). PubMed: 32917722
- Lynch JM et al. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle. PLoS One 13:e0206801 (2018). PubMed: 30395621
- Lynch JM et al. Binding of a glaucoma-associated myocilin variant to the aB-crystallin chaperone impedes protein clearance in trabecular meshwork cells. J Biol Chem 293:20137-20156 (2018). PubMed: 30389787
