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Donkey Anti-Goat IgG H&L (Biotin) preadsorbed (ab7124)

Submit a review Q&A (1)References (4)

Key features and details

  • Donkey Anti-Goat IgG H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Donkey
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Goat

  • Tested applications

    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
Notes
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/16000 - 1/64000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab7124? Please let us know so that we can cite the reference in this datasheet.

ab7124 has been referenced in 4 publications.

  • Kurosawa T  et al. A murine model of acute lung injury identifies growth factors to promote tissue repair and their biomarkers. Genes Cells 24:112-125 (2019). PubMed: 30474194
  • Tan S  et al. Altered Biological Properties in Dp71 Over-Expressing HBE Cells. Cell Physiol Biochem 43:2022-2036 (2017). PubMed: 29059680
  • Rienstra H  et al. Donor and recipient origin of mesenchymal and endothelial cells in chronic renal allograft remodeling. Am J Transplant 9:463-72 (2009). IHC-P . PubMed: 19260830
  • Broekema M  et al. Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. J Am Soc Nephrol 18:165-75 (2007). PubMed: 17135399

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

What information do you require regarding the 3 antibodies? Due to the research we are doing, we don't want to name what the antibodies are raised to, but I can give the following information:

Ab1: mouse monoclonal IgG2A, stored in PBS (pH 7.4) and Protein A and G purified

Ab2: mouse monoclonal IgG1, stored in PBS (pH 7.4) and Protein G purified

Ab3: mouse monoclonal IgG1K, stored in PBS (pH 7.4) and Protein G purified

Is there any other information you require on the coating antibodies?

Best Wishes,

Read More
Answer

Thank you for your message and for providing this further information.

Reviewing the details, I agree there seems to be some crossreactivity to mouse IgG. I can confirm that this antibody has not specifically been pre absorbedwith mouse, so we cannot guarantee it would not crossreact with mouse. However, I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note in compensation.

If you decide on a free of charge replacement, I can suggest it would be beneficial to select an alternativeantibody that has been cross absorbed with mouse, so there is minimal possible crossreactivity. For example you may like to consider the following which has been tested and guaranteed in ELISA:

ab7124 Donkey anti-Goat IgG H&L (Biotin) secondary antibody
https://www.abcam.com/Donkey-Goat-IgG-HL-Biotin-preadsorbed-ab7124.html (or use the following: https://www.abcam.com/Donkey-Goat-IgG-HL-Biotin-preadsorbed-ab7124.html).

I look forward to hearing from you with details of how you would like to proceed.

Read More

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