Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISA
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Tested applications
Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details -
Minimal
cross-reactivity
Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
Immunogen
Goat IgG, whole molecule
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Conjugation
Cy5 ®. Ex: 650nm, Em: 667nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
Conjugation notes
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent dilution.
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ELISA |
1/10000 - 1/50000.
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Notes |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/50000. |
Images
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Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 µm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 µm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.
Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).
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Lanes 2-3 : Goat anti-UCP1 at 1/200 dilution
Lane 1 : Marker
Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg
Secondary
Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution
Observed band size: 33 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab6566 has been referenced in 40 publications.
- Li H et al. Alterations of CD8+ T cells in the blood and salivary glands of patients with primary Sjögren's syndrome. Clin Rheumatol 42:1327-1338 (2023). PubMed: 36609932
- Zhao LM et al. Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells. Bioact Mater 14:206-218 (2022). PubMed: 35310356
- Ferreira JV et al. LAMP2A regulates the loading of proteins into exosomes. Sci Adv 8:eabm1140 (2022). PubMed: 35333565
- Yoshida Y et al. Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue. iScience 25:104268 (2022). PubMed: 35521515
- Won N et al. Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days. Cells 11:N/A (2022). PubMed: 35681517