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    products/secondary-antibodies/donkey-goat-igg-hl-dylight-488-ab96931.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (DyLight® 488) (ab96931)

  • Datasheet
Submit a review Q&A (2)References (14)

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Abpromise

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Donkey Anti-Goat IgG H&L (DyLight® 488) (ab96931)
  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (DyLight® 488) (ab96931)

Key features and details

  • Donkey Anti-Goat IgG H&L (DyLight® 488)
  • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: Flow Cyt, ICC/IF, IHC-P

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Overview

  • Product name

    Donkey Anti-Goat IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • DyLight® 488

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50 - 1/200.
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.
Notes
Flow Cyt
1/50 - 1/200.
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.

Images

  • Donkey Anti-Goat IgG H&L (DyLight® 488) (ab96931)
    Donkey Anti-Goat IgG H&L (DyLight® 488) (ab96931)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (DyLight® 488) (ab96931)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (DyLight® 488) (ab96931)Courtesy of an anonymous Abreview

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (14)

Publishing research using ab96931? Please let us know so that we can cite the reference in this datasheet.

ab96931 has been referenced in 14 publications.

  • Hearn JI  et al. N-Methyl-D-Aspartate Receptor Hypofunction in Meg-01 Cells Reveals a Role for Intracellular Calcium Homeostasis in Balancing Megakaryocytic-Erythroid Differentiation. Thromb Haemost 120:671-686 (2020). PubMed: 32289863
  • Jara C  et al. Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue. Diabetol Metab Syndr 12:66 (2020). PubMed: 32774470
  • El-Bazzal L  et al. Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1. Hum Mol Genet 28:2378-2394 (2019). PubMed: 31090908
  • Tang S  et al. Apolipoprotein A-I enhances insulin-dependent and insulin-independent glucose uptake by skeletal muscle. Sci Rep 9:1350 (2019). PubMed: 30718702
  • Wakasugi T  et al. Role of smooth muscle cell p53 in pulmonary arterial hypertension. PLoS One 14:e0212889 (2019). PubMed: 30807606
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Phone call requesting a secondary antibody to goat IgG which has been pre-adsorbed to reduce cross reactivity with rabbit.

Read More

Abcam community

Verified customer

Asked on Dec 05 2012

Answer

Thank you for contacting us and your interest in our products.

I am sorry for the delay in getting back to you. We have quite a few antibodies which should be suitable for your experiment and should reduce the level of non-specific staining you are currently observing. The following antibodies have all been pre-adsorbed to reduce the cross reactivity with rabbit IgG (as well as IgG from other species):

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3.5 ®), pre-adsorbed (ab6950)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5.5 ®), pre-adsorbed (ab6951)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy2 ®), pre-adsorbed (ab6948)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed (ab96935)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 594), pre-adsorbed (ab96937)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 650), pre-adsorbed (ab96938)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab97120)

If there is any other conjugate you would prefer for your experiment please let me know and I can have a look to see if we have one which has been pre-adsorbed to reduce cross reactivity with rabbit.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

Read More

Abcam Scientific Support

Answered on Dec 05 2012

Question

Thanks,

Is this biotin one also ok?

https://www.abcam.com/Goat-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7124.html#minReactivity

Best regards,

Read More

Abcam community

Verified customer

Asked on Dec 05 2012

Answer

Thank you for your reply. I'm glad my email reached you.

Yes, the donkey anti-Goat IgG H&L (Biotin) secondary antibody (ab7124) should also work fine for the experiment you are planning.

If I can be of any further help, please do let me know. Until then, I wish you all the best with your research.

Read More

Abcam Scientific Support

Answered on Dec 05 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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