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Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed (ab96938)

Reviews (1)Q&A (1)References (8)
Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed (ab96938)

    Key features and details

    • Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed
    • Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 25nm HRP HRP polymer PE Texas Red ® TRITC Unconjugated Unconjugated

    Overview

    • Product name

      Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed
      See all IgG secondary antibodies

    • Host species

      Donkey

    • Target species

      Goat

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

       

    • Tested applications

      Suitable for: IHC-P, ICC/IF, Flow Cytmore details

    • Minimal
      cross-reactivity


      Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details

    • Conjugation

      DyLight® 650. Ex: 654nm, Em: 673nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • General notes

      DyLight® 650 replaces DyLight® 649.

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96938 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    Images

    • Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed (ab96938)
      Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed (ab96938)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (8)

    Publishing research using ab96938? Please let us know so that we can cite the reference in this datasheet.

    ab96938 has been referenced in 8 publications.

    • Crawley-Snowdon H  et al. Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29. Nat Commun 11:5031 (2020). PubMed: 33024112
    • Kathe C & Moon LDF RNA sequencing dataset describing transcriptional changes in cervical dorsal root ganglia after bilateral pyramidotomy and forelimb intramuscular gene therapy with an adeno-associated viral vector encoding human neurotrophin-3. Data Brief 21:377-385 (2018). PubMed: 30364576
    • Scherz B  et al. mTh1 driven expression of hTDP-43 results in typical ALS/FTLD neuropathological symptoms. PLoS One 13:e0197674 (2018). PubMed: 29787578
    • Rodriguez AM & Downs KM Visceral endoderm and the primitive streak interact to build the fetal-placental interface of the mouse gastrula. Dev Biol N/A:N/A (2017). PubMed: 28882402
    • Kullmann FA  et al. Urothelial proliferation and regeneration after spinal cord injury. Am J Physiol Renal Physiol 313:F85-F102 (2017). PubMed: 28331065
    • Baron L  et al. Mycolactone subverts immunity by selectively blocking the Sec61 translocon. J Exp Med 213:2885-2896 (2016). PubMed: 27821549
    • Kathe C  et al. Intramuscular Neurotrophin-3 normalizes low threshold spinal reflexes, reduces spasms and improves mobility after bilateral corticospinal tract injury in rats. Elife 5:N/A (2016). Rat . PubMed: 27759565
    • Rönnlund D  et al. Multicolor fluorescence nanoscopy by photobleaching: concept, verification, and its application to resolve selective storage of proteins in platelets. ACS Nano 8:4358-65 (2014). PubMed: 24730587

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Stained for MPO (primary) in injured rat spinal cord tissue with DyLight 650

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Good secondary, showed up well with 1:500
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 07 2021

    Question

    Customer inquired about detecting presence of 7 different proteins in brain tissue via IHC-F. Would like 3-4 different host species for primary, secondary antibodies that do not overlap in spectra. Customer is also not sure if Caspase 3, Nogo, and neurofilament are necessary for detecting apoptosis / neuronal response.

    Read More
    Answer

    Thank you for contacting Abcam.

    I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numerous specific published references. The list for these 4 antibodies is given below:
    1. GFAP ab4674 (https://www.abcam.com/gfap-antibody-astrocyte-marker-ab4674.html)
    2. FOX3 ab104225 (https://www.abcam.com/fox3neun-antibody-ab104225.html)
    3. Iba1 ab5076 (https://www.abcam.com/iba1-antibody-ab5076.html)
    4. CD68 ab31630 (https://www.abcam.com/cd68-antibody-ed1-ab31630.html)

    I also compiled anti-chicken, rabbit, goat, and mouse secondaries with separate emission spectra. We don't have secondaries for these species that weren't raised in any of the 4 species conjugated to a fluorescent protein with a similar spectrum to DAPI. Thus, I think your best bet is to obtain a far-red filter (Cy5), that way you could still stain with DAPI as well. The donkey secondaries that I found with separate emission spectra are listed below:
    1. anti-chicken: FITC (https://www.abcam.com/chicken-igy-secondary-antibody-ab63507.html)
    2. anti-rabbit: DyLight 594 (Similar spectrum to Texas Red) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-rabbit-igg-h-l-dylight-594-pre-adsorbed-ab96921.html)
    3. anti-goat: DyLight 650 (Similar spectrum to Cy5) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-h-l-dylight-650-pre-adsorbed-ab96938.html)
    4. anti-mouse: DyLight 550 (similar spectrum to TRITC) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-mouse-igg-h-l-dylight-550-ab96876.html)

    The other antibodies you chose (Nogo, heavy neurofilament, caspase 3) seem to be good choices as well as they are tested in formaldehyde fixed, frozen sections via IHC in rat. Below are the antibodies we discussed against those three proteins:
    1. anti-Nogo (https://www.abcam.com/nogo-antibody-ab32298.html)
    2. anti-heavy neurofilament (https://www.abcam.com/200-kd-neurofilament-heavy-antibody-ab134459.html)
    3. anti-caspase 3 (https://www.abcam.com/active-caspase-3-antibody-ab13847.html)

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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