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Donkey Anti-Goat IgG H&L (FITC) (ab6881)

Submit a review Q&A (3)References (48)
Flow Cytometry - Donkey Anti-Goat IgG H&L (FITC) (ab6881)
  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (FITC) (ab6881)

Key features and details

  • Donkey Anti-Goat IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, IHC-Fr, ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (FITC)
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Goat

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, WBmore details

  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
WB
1/5000 - 1/20000.
Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
WB
1/5000 - 1/20000.

Images

  • Flow Cytometry - Donkey Anti-Goat IgG H&L (FITC) (ab6881)
    Flow Cytometry - Donkey Anti-Goat IgG H&L (FITC) (ab6881)

    ab6881 was used at dilution 1/200 with the primary antibody ab27985 in Flow Cyt. See the review on ab27985.

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (FITC) (ab6881)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (FITC) (ab6881)

    ab6881 was used at dilution 1/500 with the primary antibody ab26234 in ICC/IF. See the review on ab26234.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (48)

Publishing research using ab6881? Please let us know so that we can cite the reference in this datasheet.

ab6881 has been referenced in 48 publications.

  • Su Y  et al. Exosomes derived from placental trophoblast cells regulate endometrial epithelial receptivity in dairy cows during pregnancy. J Reprod Dev 68:21-29 (2022). PubMed: 34690214
  • Wang Z  et al. Clock Gene Nr1d1 Alleviates Retinal Inflammation Through Repression of Hmga2 in Microglia. J Inflamm Res 14:5901-5918 (2021). PubMed: 34795498
  • Yue D  et al. EZH2 inhibitor GSK343 inhibits sepsis-induced intestinal disorders. Exp Ther Med 21:437 (2021). PubMed: 33747174
  • McGuirk S  et al. Resistance to different anthracycline chemotherapeutics elicits distinct and actionable primary metabolic dependencies in breast cancer. Elife 10:N/A (2021). PubMed: 34181531
  • Inoue H  et al. The zinc-binding motif of TRPM7 acts as an oxidative stress sensor to regulate its channel activity. J Gen Physiol 153:N/A (2021). PubMed: 33999118
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

Madame, Monsieur,
Pourriez-vous me faire parvenir un devis incluant tous frais annexes (taxes, frais de port...) pour l'article suivant : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (FITC) réf. ab6881.

Je souhaiterais également savoir si vous disposez d'informations quant à son utilisation en Western Blot. Pouvez-vous me faire parvenir un échantillon afin de le tester dans cette application ?
En vous remerciant par avance,
Cordialement,

Read More
Answer

Merci de nous avoir contactés.

Vous avez du recevoir dans un autre email un devis pour l'anticorps ab6881.

J'ai reçu confirmation du laboratoire que l'anticorps ab6881 fonctionne en Western Blot. Les dilutions recommandées sont 1/5000 - 1/20000. La fiche technique a été mise à jour avec ces informations (https://www.abcam.com/ab6881).

Comme nous avons plus de 90.000 références, nous ne pouvons stocker différents volumes pour nos produits et nous n'offrons donc pas d'échantillon gratuit ou de petit volume pour des essais préliminaires.

Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique, nous offrons un remboursement ou un remplacement gratuit.

J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

Read More

Question

Thank you for your responce I have attached the protocol that we use The lot number for the secondary was lot63139 and the cat no. it ab6881-1 I do not know the exact time it took for the order but the product was order by the Brombacher lab at University of Cape Town, Infectious disease unit Please lte me know if you need more info. Thanks for your time

Read More
Answer

Thank you for your protocol and your order information, we have sold only 1 vial of anti-Foxp3 to a customer in Cape town but the address is very different and it was bought in March, could this be you? I have carefully looked at each step of your protocol and would recommend the following modifications: -you say "defrost after fixing" and then mention fixing again. Can I please make sure the sections are fresh frozen and not perfusion fixed. Fresh frozen sections should be then postfixed for 20min in 4% paraformaldehyde in PB, or for 10min in ice cold acetone. -I recommend washing slides in PBS 3-4 times between each step, to decrease non specific binding -use the dilution buffer PBST to dilute the blocking agent and antibodies. PBST is made of PBS + 0.3% v/v TritonX100, to permeabilise the cells. Even if your sections are very thin they may need permeabilisation (if you fix with acetone this is not necessary as acetone fixes and permeabilises too) -block in normal serum 10% rather than BSA, this can give problems in some rare cases - try to use biotin-ABC-DAB rather than IF, as this is more specific and more sensitive. I hope these suggestions will help you, please let me know how you get on and good luck with your experiments,

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Question

Need information on: FOXP3 antibody cat. ab2481 Abcam Goat IgG antibody (ab6881) datasheet 1 mg cat. ab6881 Abcam I need to know what kind of resultS can i obtain if we use them with PERCP or SYCRON . I dont see any information in your website ... We hope you can help me with this please If you have any questions please feel to call at any time,,

Read More
Answer

Thank you for your email. For PERCP, do you mean the tandem conjugate PerCP-Cy5.5? I'm not sure what you mean by Sycron so it would great if you could provide me with some more information.

Read More

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