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  1. Link

    products/secondary-antibodies/donkey-goat-igg-hl-hrp-ab97110.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (HRP) (ab97110)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (58)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)

Key features and details

  • Donkey Anti-Goat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, WB, ICC

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Overview

  • Product name

    Donkey Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Immunogen

    Other Immunogen Type corresponding to Goat IgG. Contains both the heavy and light chain

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Constituent: 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Part of the AbExcel range.
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Enzyme
    • HRP

Associated products

  • Related Products

    • Normal Donkey Serum (ab7475)
  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200 - 1/500.
ELISA (1)
1/10000 - 1/100000. (Primary).
WB (3)
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC
1/200 - 1/500.
Notes
IHC-P
1/200 - 1/500.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC
1/200 - 1/500.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 & 5-6 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution
    Lanes 3-4 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 minutes

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (58)

Publishing research using ab97110? Please let us know so that we can cite the reference in this datasheet.

ab97110 has been referenced in 58 publications.

  • Kumar V  et al. Dual Specific Phosphatase 14 Deletion Rescues Retinal Ganglion Cells and Optic Nerve Axons after Experimental Anterior Ischemic Optic Neuropathy. Curr Eye Res 46:710-718 (2021). PubMed: 33107352
  • Liu H  et al. Erb-B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53-responsive microRNA-3184-5p in cervical cancer cells. Oncol Rep 45:95-106 (2021). PubMed: 33416166
  • Sui Q  et al. Dickkopf 1 impairs the tumor response to PD-1 blockade by inactivating CD8+ T cells in deficient mismatch repair colorectal cancer. J Immunother Cancer 9:N/A (2021). PubMed: 33782107
  • Burchill MA  et al. Oxidized Low-Density Lipoprotein Drives Dysfunction of the Liver Lymphatic System. Cell Mol Gastroenterol Hepatol 11:573-595 (2021). PubMed: 32961356
  • Shi X  et al. Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro. J Cell Mol Med 25:2563-2571 (2021). PubMed: 33523599
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Direct ELISA method development

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Capture : Purified recombinant protein
1st Detection : Anti-target Goat Ab (1:5000)
2nd Detection : Donkey Anti-goat IgG H&L (1:6000)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 17 2020

Excellent 2nd antibody for WB

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab166930 (1:2000).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Baptiste Jude

Verified customer

Submitted Jul 06 2016

excellent antibody for WB assay

Excellent
Abreviews
Abreviews
Application
Western blot
This antibody is good for western blot assay, a dilution of 1 to 5,000 could always offer a good detection signal, which was comparable to the dilution of 1 to 2,000.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 05 2015

good 2nd Ab for WB

Excellent
Abreviews
Abreviews
Application
Western blot
a good 2nd Ab for WB (1:5,000 dilution).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2015

Question

I have been using ab97110 as a secondary for my Westerns. However, since we only need such a amount and very dilute, we end up wasting a lot of it. I only am running Westerns semi-regularly and the workload comes in waves, so it possible to aliquot and freeze the antibody for long-term storage? If so, at -20 or -80? Thank you. Sincerely, \

Read More

Abcam community

Verified customer

Asked on Apr 23 2013

Answer

We have studied the effect of freeze thaw cycles on conjugated antibodies and have seen that they can have significant effect on HRP secondaries. As such we do not recommend stroage at -20C. These products should have good activity for one year or more when stored at +4C and are covered by our 12 month Abpromise guarantee.

Read More

Abcam Scientific Support

Answered on Apr 23 2013

Question

Sir/ Madam, Sorry to disturb you. Our customer makes a request about ab97110:Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (lot:gr13665-10). He has ordered this antibody recently, and he would like to get the COC. Would you please help him provide the COC? Thank you very much :) Best regards.

Read More

Abcam community

Verified customer

Asked on Jan 04 2012

Answer

Thank you for your enquiry. Please find enclosed as requested the CoC for ab97110, lot number GR13665-10. I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

Read More

Abcam Scientific Support

Answered on Jan 04 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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