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Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab97120)

Reviews (2)Q&A (4)References (24)

Key features and details

  • Donkey Anti-Goat IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Goat

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Human, Mouse, Pig, Rabbit, Rat more details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000. (Primary)
WB (2)
1/5000 - 1/50000. Colorimetric 1/5000 - 1/30000; Chemiluminescent 1/10000 - 1/50000
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000. (Primary)
WB
1/5000 - 1/50000. Colorimetric 1/5000 - 1/30000; Chemiluminescent 1/10000 - 1/50000

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (24)

Publishing research using ab97120? Please let us know so that we can cite the reference in this datasheet.

ab97120 has been referenced in 24 publications.

  • Kobayashi H  et al. The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis. Gastroenterology 160:1224-1239.e30 (2021). PubMed: 33197448
  • Meßner M  et al. Small molecule inhibitors of the mitochondrial ClpXP protease possess cytostatic potential and re-sensitize chemo-resistant cancers. Sci Rep 11:11185 (2021). PubMed: 34045646
  • Pomierny B  et al. The Slow-Releasing and Mitochondria-Targeted Hydrogen Sulfide (H2S) Delivery Molecule AP39 Induces Brain Tolerance to Ischemia. Int J Mol Sci 22:N/A (2021). PubMed: 34360581
  • Huang Y  et al. Function of miR-200a in proliferation and apoptosis of non-small cell lung cancer cells. Oncol Lett 20:1256-1262 (2020). PubMed: 32724366
  • Siljee S  et al. Cancer Stem Cells in Head and Neck Metastatic Malignant Melanoma Express Components of the Renin-Angiotensin System. Life (Basel) 10:N/A (2020). PubMed: 33147716
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

works fine

 Excellent
Abreviews
abreview image
Application
Western blot
Recognizes primary raised in goat, works well in 1:4000-5000 dilutions
incubated for 1 hour in 5% BSA
lanes 1,2,3 are replicates
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 30 2019

the Donkey Anti Goat 2ry works fine

 Excellent
Abreviews
Application
Western blot
the antibody works fine and identifies the primaries that are raised in goat.
Experimental conditions: Dilution 1:2000 in 5% BSA.
incubated in room temperature for 2 hours
washed in TTBS 3 times for 10 minutes before and after the secondary.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 01 2019

Question

For antibody ab97120 you mention that the antibody is minimally cross reactive to chicken, horse, human, mouse, pig, rabbit and rat IgGs. But what about sheep IgG? I'm looking for an antibody that I can use in a sandwich ELISA that only binds to goat IgG and not sheep IgG.

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Answer



I am happy to confirm that we have a anti goat antibody that was pre-absorped against sheep IgG:

ab99713

https://www.abcam.com/Mouse-monoclonal-SB115d-Goat-IgG-Fc-HRP-preadsorbed-ab99713.html (or use the following: https://www.abcam.com/Mouse-monoclonal-SB115d-Goat-IgG-Fc-HRP-preadsorbed-ab99713.html).

Please review the datasheet for more information.

This antibody is tested and guaranteed for ELISA as well.

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Question

I have a request for a certificate of analysis for AB97120, lot # is GR62374-4.

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Answer

Thank you for contacting Abcam.


Attached please find the CoC document you requested.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Question

Dear Tech Support Team, Please see below the details of customer's complaint. Attached is the image of the gel. Please advise. Antibody code: Ab9134 Batch number: Gr4307-5 Antibody storage conditions (temperature/reconstitution etc) 40C Description of the problem (high background, wrong band size, more bands, no band etc.) We got a strong band of 90kDa and weak band of the protein with the HA- tag and in the negative control (lymphocytes) we got just the wrong band. We also checked the secondary antibody against the SDS PAGE without the first antibody and the blot was clean. Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) Lymphocytes cell extract, nuclear and cytoplasmatic extract of HEK 293 cells with the HA tag over-expression   Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) The cells lysed in RIPA buffer of 50 mM Tris pH 8.0, 150 mM NaCl, 5 mM EDTA pH 8.0, 1% NP-40, 0.5% Sodium deoxycholate (DOC), 0.1% SDS and incubated on ice for 30 min. Loading buffer (10% SDS , 10mM beta-mercapto-ethanol,20 %  Glycerol, 0.2 M Tris-HCl, pH 6.8, 0.05% Bromophenolblue) added to the samples and they boiled for 10 minutes and loaded on the gel.   Amount of protein loaded 167000 cells per well Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) The samples run on the gel of 4-15% Mini protean TGX –BioRAD. Transfer and blocking conditions (Buffer/time period, Blocking agent etc.) The gel transferred for 90 min. on 150 mA in transfer buffer (25 mM Tris-HCl, 192 mM  Glycine, 0.1% SDS, 20% Methanol) and blocked in 1% skim milk with 0.3% Tween-20 in PBS, for an hour at room temperature. Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) The antibody diluted 1:1000 in 1% skim milk with 0.3% Tween-20 in PBS, over night at 40C.  The membrane washed three times with PBS with 0.3% Tween-20.  Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) The secondary antibody donkey polyclonal to Goat IgG (ab97120) diluted 1:12000 in 1% skim milk with 0.3% Tween-20 in PBS and incubated at room temperature for an hour. The membrane washed three times with PBS with 0.3% Tween-20.    Detection method (ECL, ECLPlus etc.) SuperSignal westpico chemiluminescent substrate (Pierce) used as a detection. Positive and negative controls used (please specify) We used the lymphocytes as a negative control but we got the wrong band on them also. We didn’t use any positive control.   Optimization attempts (problem solving)   How many times have you tried the Western? Twice Have you run a "No Primary" control? No we didn’t use that control but we used the same blot with the primary antibody of ab96326. Do you obtain the same results every time? e.g. are the background bands always in the same place? Yes What steps have you altered? We used different over-expression with the HA-tag.  

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Answer

Thank you for contacting us. I have read the whole protocol you have kindly submitted. In order to address the problem quickly I have further questions to ask. - According to the attached image you have used non transfected N2a mouse cells, Lymphocytes supernatant and Lymphocytes pellet as a negative control right? I am sorry I am surprised by the fact that 1st and 3rd (very very faint band may due to spillage from 4th lane) there is no band while there is dark band in 2nd lane. Could you confirm there was no intermixing of lysates? - What is the band size you are expecting to be? - The description of the problem also does not correlate with the image. Does this mean the Lymphocyte pellet has HA-tag inserted cells? I predict from the image; the problem only is that antibody is detecting a band in Lymphocyte supernatant which it should not do. Right? - Could you specify which protein you have tagged with HA? Have you done any western blot studies with anti protein antibody? - Could you provide the order details so that I check if the delivery was on time? I will look forward to hearing from you soon.

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Question

I am trying to find the material safety data sheets for the following products.  Please advise if no MSDS is required. Anti-MUC7 Antibody (4D2-1D7) AB105466 Donkey Polyclonal Secondary Antibody to Goat IgG - H&L (HRP) AB97120 Rabbit Polyclonal Secondary antibody to Goat IgG-H&L (HRP) AB97105

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Answer

Thank you for contacting Abcam.   I have attached the MSDS for ab97120 and ab97105, both of which contain the preservative proclin.  A MSDS is not required for ab105466.   I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

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