Donkey Anti-Goat IgG H&L (TRITC) (ab6882)
Key features and details
- Donkey Anti-Goat IgG H&L (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Donkey
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISA
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FITC
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PE
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Unconjugated
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Overview
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Product name
Donkey Anti-Goat IgG H&L (TRITC)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details -
Immunogen
Full length native Goat IgG (purified).
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Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.6 moles TRITC per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| IHC-P | ||
| IHC-Fr | ||
| Immunomicroscopy | ||
| Flow Cyt | ||
| ELISA |
| Notes |
|---|
Application notes
Flow Cyt: Use at an assay dependant dilution.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (4)
ab6882 has been referenced in 4 publications.
- He T et al. Inhibition of two-pore channels in antigen-presenting cells promotes the expansion of TNFR2-expressing CD4+Foxp3+ regulatory T cells. Sci Adv 6:N/A (2020). PubMed: 32998896
- Reyes-Miguel T et al. CDC42 drives RHOA activity and actin polymerization during capacitation. Reproduction 160:393-404 (2020). PubMed: 32567555
- Sun X et al. Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia. Exp Ther Med 15:4709-4716 (2018). PubMed: 29805490
- Tao X et al. In vitro examination of microglia-neuron crosstalk with BV2 cells, and primary cultures of glia and hypothalamic neurons. Heliyon 4:e00730 (2018). PubMed: 30148218
