Donkey Anti-Human IgG H&L preadsorbed (ab102421)
Key features and details
- Donkey Anti-Human IgG H&L preadsorbed
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Donkey Anti-Human IgG H&L preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details -
Immunogen
Other Immunogen Type corresponding to IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/30000. ELISA - Coating: 1/100 - 1/500; ELISA - Primary: 1/1000 - 1/30000
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|
WB |
1/1000 - 1/30000. WB - Colorimetric: 1/1000 - 1/10000; WB - Chemiluminescent: 1/1000 - 1/30000
|
Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/30000. ELISA - Coating: 1/100 - 1/500; ELISA - Primary: 1/1000 - 1/30000 |
WB
1/1000 - 1/30000. WB - Colorimetric: 1/1000 - 1/10000; WB - Chemiluminescent: 1/1000 - 1/30000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab102421 has been referenced in 1 publication.
- Henson D et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438