Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175472)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568)
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ELISA, Flow Cyt, ICC/IF, IHC-P
Overview
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Product name
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: IHC-Fr, ELISA, Flow Cyt, ICC/IF, IHC-Pmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| ICC/IF | (1) |
1/200 - 1/1000.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
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ICC/IF
1/200 - 1/1000. |
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IHC-P
Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175472)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175472 Alexa Fluor® 568 donkey anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175472)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab175472 has been referenced in 33 publications.
- Leduc-Pessah H et al. Spinal A3 adenosine receptor activation acutely restores morphine antinociception in opioid tolerant male rats. J Neurosci Res 100:251-264 (2022). PubMed: 34075613
- Rudnitskaya EA et al. Changes in Glial Support of the Hippocampus during the Development of an Alzheimer's Disease-like Pathology and Their Correction by Mitochondria-Targeted Antioxidant SkQ1. Int J Mol Sci 23:N/A (2022). PubMed: 35163053
- Gaudin N et al. Evolutionary conservation of centriole rotational asymmetry in the human centrosome. Elife 11:N/A (2022). PubMed: 35319462
- Rütsche D et al. The Role of CD200-CD200 Receptor in Human Blood and Lymphatic Endothelial Cells in the Regulation of Skin Tissue Inflammation. Cells 11:N/A (2022). PubMed: 35326506
- Dey MR et al. Granule Cells Constitute One of the Major Neuronal Subtypes in the Molecular Layer of the Posterior Cerebellum. eNeuro 9:N/A (2022). PubMed: 35584915