Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISA
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISAmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000 - 1/4000.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
1/2000 - 1/4000. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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ELISA
Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150107 Alexa Fluor® 647 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Donkey anti-mouse IgG H&L (Alexa Fluor® 647) (ab150107) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.
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Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)Emmission spectra of the Alexa Fluor® conjugated secondary antibodies.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (93)
ab150107 has been referenced in 93 publications.
- Miesen L et al. Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis. Dis Model Mech 15:N/A (2022). PubMed: 34927672
- Aldrin-Kirk P et al. A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants. Stem Cell Reports 17:159-172 (2022). PubMed: 34971563
- Theocharidis G et al. Single cell transcriptomic landscape of diabetic foot ulcers. Nat Commun 13:181 (2022). PubMed: 35013299
- Weichseldorfer M et al. Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies. J Transl Med 20:39 (2022). PubMed: 35073923
- Li W et al. Machine Learning-Assisted Dual-Marker Detection in Serum Small Extracellular Vesicles for the Diagnosis and Prognosis Prediction of Non-Small Cell Lung Cancer. Nanomaterials (Basel) 12:N/A (2022). PubMed: 35269297