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Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7062)

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Key features and details

  • Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Donkey
  • Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.

  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
Notes
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/500 - 1/2000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab7062? Please let us know so that we can cite the reference in this datasheet.

ab7062 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Product code: 7062

Inquiry: The customer wishes to use ab7062 as a secondary to ab24470. Do you predict some problem with it (since ab24470 is already conjugated to HRP)? Thanks in advance for your advise. Have a nice week.

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Answer

Thank you for your inquiry.
There may be a problem in using the primary antibody ab24470 with a secondary antibody as it is already HRP conjugated itself. The steric hindrance created by this conjugate may hinder the efficient binding of the secondary antibody and may therefore lead to a reduced signal in the assay. As we have not tried this ourselves we cannot know exatly how badly the signal will be affected.
I would recommend using an unconjugated antibody such as ab8315 or ab14729 as these will not lead to such problems.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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