Donkey Anti-Mouse IgG H&L (Biotin) (ab6819)
Key features and details
- Donkey Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF
Overview
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Product name
Donkey Anti-Mouse IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details -
Immunogen
mouse IgG whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | ||
| ELISA | ||
| IHC-P | ||
| IHC-Fr | ||
| Immunomicroscopy | ||
| WB | ||
| ICC/IF |
| Notes |
|---|
Application notes
immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab6819 has been referenced in 1 publication.
- Adebiyi OE et al. Sodium metavanadate induced cognitive decline, behavioral impairments, oxidative stress and down regulation of myelin basic protein in mice hippocampus: Ameliorative roles of ß-spinasterol, and stigmasterol. Brain Behav 8:e01014 (2018). PubMed: 29856129