Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060)
Key features and details
- Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Donkey
- Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy
Overview
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Product name
Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details -
Minimal
cross-reactivity
Cow, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native mouse IgG (purified).
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
ICC/IF | ||
Dot blot | ||
ELISA | ||
IHC-P | ||
IHC-Fr | ||
Immunomicroscopy |
Notes |
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ELISA: 1/50000 - 1/200000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab7060 has been referenced in 2 publications.
- Korrell KV et al. Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse. J Anat N/A:N/A (2019). PubMed: 30901089
- Hoerder-Suabedissen A & Molnár Z Molecular Diversity of Early-Born Subplate Neurons. Cereb Cortex : (2012). PubMed: 22628460