Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)
Key features and details
- Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
Overview
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Product name
Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/50 - 1/200.
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ICC/IF |
1/40 - 1/500.
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IHC-P |
1/40 - 1/400.
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WB |
1/1000 - 1/20000. Predicted molecular weight: 36 kDa.
5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended. |
Notes |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/40 - 1/500. |
IHC-P
1/40 - 1/400. |
WB
1/1000 - 1/20000. Predicted molecular weight: 36 kDa. 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended. |
Images
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All lanes : Cocktail of mouse anti-hnRNP, rabit anti-actin and goat anti-GAPDH at 1 µg
Lane 1 : HeLa whole cell lysate at 30 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Secondary
All lanes : Cocktail containing ab98794 donkey anti-mouse Dylight 488 (blue); donkey anti-rabbit Dylight 680 (green) and donkey anti-goat Dylight 800 (red) at 0.5 µg
Predicted band size: 36 kDaAcquisition: Syngene G:Box, 34 seconds (blue), 20 seconds (green), and 50 seconds (red)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)
Immunohistochemistry immunofluorescent analysis of human ovarian carcinoma tissue using primary mouse anti-p53 at 1/100 dilution and secondary ab98794 at 1/50 with different lots on left and right panels. Counterstain was DAPI.
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab98794 has been referenced in 5 publications.
- Otte K et al. Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide. Biomedicines 10:N/A (2022). PubMed: 36140245
- Hinteregger B et al. Metabolic, Phenotypic, and Neuropathological Characterization of the Tg4-42 Mouse Model for Alzheimer's Disease. J Alzheimers Dis 80:1151-1168 (2021). PubMed: 33646155
- Zhao K et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells. Int J Mol Sci 23:N/A (2021). PubMed: 35008590
- Shir A et al. S101, an Inhibitor of Proliferating T Cells, Rescues Mice From Superantigen-Induced Shock. J Infect Dis 217:288-297 (2018). PubMed: 29149330
- Gunness P et al. 3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies. Toxicol Sci 133:67-78 (2013). PubMed: 23377618