Donkey Anti-Mouse IgG H&L (HRP) (ab97030)
Key features and details
- Donkey Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Donkey Anti-Mouse IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
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| IHC-P |
1/200 - 1/5000.
|
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| ELISA |
1/1000 - 1/100000. (Primary)
|
|
| WB |
1/5000 - 1/30000. (Colorimetric) 1/1000 - 1/50000 (Chemiluminescent)
|
| Notes |
|---|
|
ICC
Use at an assay dependent dilution. |
|
IHC-P
1/200 - 1/5000. |
|
ELISA
1/1000 - 1/100000. (Primary) |
|
WB
1/5000 - 1/30000. (Colorimetric) 1/1000 - 1/50000 (Chemiluminescent) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab97030 has been referenced in 9 publications.
- Zhao K et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells. Int J Mol Sci 23:N/A (2021). PubMed: 35008590
- Zhao W et al. MicroRNA-154-5p regulates the HPV16 E7-pRb pathway in Cervical Carcinogenesis by targeting CUL2. J Cancer 11:5379-5389 (2020). PubMed: 32742484
- Pan S et al. Convergent genomic signatures of flight loss in birds suggest a switch of main fuel. Nat Commun 10:2756 (2019). PubMed: 31227702
- Lian HY et al. Generation and propagation of yeast prion [URE3] are elevated under electromagnetic field. Cell Stress Chaperones 23:581-594 (2018). PubMed: 29214607
- Zhang L et al. ATM-JAK-PD-L1 signaling pathway inhibition decreases EMT and metastasis of androgen-independent prostate cancer. Mol Med Rep 17:7045-7054 (2018). PubMed: 29568923
- Bischoff FC et al. Identification and Functional Characterization of Hypoxia-Induced Endoplasmic Reticulum Stress Regulating lncRNA (HypERlnc) in Pericytes. Circ Res 121:368-375 (2017). PubMed: 28611075
- Lin CH & Lin CC Sitagliptin attenuates in?ammatory responses in lipopolysaccharide-stimulated cardiomyocytes via nuclear factor-?B pathway inhibition. Exp Ther Med 11:2609-2615 (2016). PubMed: 27284355
- Shi Z et al. The Energy Metabolism in Caenorhabditis elegans under The Extremely Low-Frequency Electromagnetic Field Exposure. Sci Rep 5:8471 (2015). WB . PubMed: 25683579
- Adderley SP et al. Histamine activates p38 MAP kinase and alters local lamellipodia dynamics, reducing endothelial barrier integrity and eliciting central movement of actin fibers. Am J Physiol Cell Physiol 309:C51-9 (2015). PubMed: 25948734