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Donkey Anti-Mouse IgG H&L (HRP) preadsorbed (ab7061)

Submit a review Q&A (2)References (6)

Key features and details

  • Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Donkey
  • Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/20000.
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/20000 - 1/80000.
IHC-P
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Notes
WB
1/2000 - 1/20000.
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/20000 - 1/80000.
IHC-P
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (6)

Publishing research using ab7061? Please let us know so that we can cite the reference in this datasheet.

ab7061 has been referenced in 6 publications.

  • Jiang S  et al. Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis. J Cell Mol Med 25:3437-3448 (2021). PubMed: 33675119
  • Jermusyk A  et al. A 584 bp deletion in CTRB2 inhibits chymotrypsin B2 activity and secretion and confers risk of pancreatic cancer. Am J Hum Genet 108:1852-1865 (2021). PubMed: 34559995
  • Fan J  et al. Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine ? lyase S-sulfhydration in ApoE-knockout atherosclerotic mice. Br J Pharmacol 176:3180-3192 (2019). PubMed: 31140595
  • Li L  et al. Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy. Exp Ther Med 18:4313-4321 (2019). PubMed: 31777538
  • Kolyada A  et al. Soluble analog of ApoER2 targeting beta2-glycoprotein I in immune complexes counteracts hypertension in lupus-prone mice with spontaneous antiphospholipid syndrome. J Thromb Haemost 14:1298-307 (2016). PubMed: 26990752
  • Kolyada A  et al. Inhibition of thrombotic properties of persistent autoimmune anti-ß2GPI antibodies in the mouse model of antiphospholipid syndrome. Blood 123:1090-7 (2014). PubMed: 24277078

Customer reviews and Q&As

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Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

I am looking for a HRP conjugated anti-mouse antibody to be used in ELISA. The most important thing is that it will have the least cross reactivity to rat immunoglobulins. I see that you have many such antibodies. Which one would you think is the most suitable?


Thanks,

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Answer

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

1. Details of preabsorbtion information of secondary ab7061. 2. Details of dilution range. Persoxidase activity block

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Answer

Thank you for your enquiry. I am sorry to hear that you have been obtaining high background with this antibody. Following contact with the source of this antibody I have determined that the antibody is affinity purified, then cross adsorbed using solid phase adsorption. This means the affinity purified antibody is passed through column(s) bound with listed species of IgGs. The antibodies which cross react with the listed IgGs will bind to the column(s), thus being removed from the final product. The major background in IHC and FACS is from Fc receptors on the cell surface. I think the best way around this problem is to use antibodies specific to the F(ab')2 fragment instead of whole IgG. e.g. ab5766 Mouse IgG antibody H&L - F(ab)2 Fragment Texas Red conjugated, Goat, FACS, IM. I do also think that it is worth inhibiting the peroxidase activity of this enzyme to ensure that the signal that you are observing is not due to the HRP substrate reacting with the endogenous peroxidase activity. I have determined from the source of this antibody that a good starting dilution is 1:1000. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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