Donkey Anti-Mouse IgG H&L (HRP) preadsorbed (ab7061)
Key features and details
- Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Donkey
- Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native mouse IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/20000.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ELISA |
1/20000 - 1/80000.
|
|
IHC-P |
1/1000 - 1/5000.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
Immunomicroscopy |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/2000 - 1/20000. |
ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent concentration. |
ELISA
1/20000 - 1/80000. |
IHC-P
1/1000 - 1/5000. |
IHC-Fr
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab7061 has been referenced in 6 publications.
- Jiang S et al. Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis. J Cell Mol Med 25:3437-3448 (2021). PubMed: 33675119
- Jermusyk A et al. A 584 bp deletion in CTRB2 inhibits chymotrypsin B2 activity and secretion and confers risk of pancreatic cancer. Am J Hum Genet 108:1852-1865 (2021). PubMed: 34559995
- Fan J et al. Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine ? lyase S-sulfhydration in ApoE-knockout atherosclerotic mice. Br J Pharmacol 176:3180-3192 (2019). PubMed: 31140595
- Li L et al. Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy. Exp Ther Med 18:4313-4321 (2019). PubMed: 31777538
- Kolyada A et al. Soluble analog of ApoER2 targeting beta2-glycoprotein I in immune complexes counteracts hypertension in lupus-prone mice with spontaneous antiphospholipid syndrome. J Thromb Haemost 14:1298-307 (2016). PubMed: 26990752
- Kolyada A et al. Inhibition of thrombotic properties of persistent autoimmune anti-ß2GPI antibodies in the mouse model of antiphospholipid syndrome. Blood 123:1090-7 (2014). PubMed: 24277078