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  1. Link

    products/secondary-antibodies/donkey-rabbit-igg-hl-10nm-gold-ab39597.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Rabbit IgG H&L (10nm Gold) (ab39597)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

Product price, shipping and contact information

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Key features and details

  • Donkey Anti-Rabbit IgG H&L (10nm Gold)
  • Conjugation: Gold 10nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: Electron Microscopy

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 12nm Gold 18nm Gold 25nm Gold 6nm HRP IRDye® 680RD Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (10nm Gold)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Tested applications

    Suitable for: Electron Microscopymore details
  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation

    Gold 10nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Gold

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Notes
Application notes
Electron Microscopy: Use at an assay dependent dilution.
Light Microscopy: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab39597? Please let us know so that we can cite the reference in this datasheet.

ab39597 has been referenced in 2 publications.

  • Müller WEG  et al. Polyphosphate as a donor of high-energy phosphate for the synthesis of ADP and ATP. J Cell Sci 130:2747-2756 (2017). PubMed: 28687622
  • Goupil LS  et al. Cysteine and Aspartyl Proteases Contribute to Protein Digestion in the Gut of Freshwater Planaria. PLoS Negl Trop Dis 10:e0004893 (2016). PubMed: 27501047

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Concentration d'anticorps?
Rapport nombre d'anticorps par bille d'or?

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Abcam community

Verified customer

Asked on Jul 05 2013

Answer

Le secondaire ab39597 couplé aux billes d'or de 10nm a les caratéristiques suivantes :

3.3 x 1011 particules d'or / ml
115-180 anticorps / bille d'or
La concentration d'anticorps est d'environ 10 µg/ml

Read More

Guillaume Boucher

Abcam Scientific Support

Answered on Jul 05 2013

Question

Plus de détails sur le ab39597

Read More

Abcam community

Verified customer

Asked on Jun 18 2013

Answer


1. ces réactifs n'ont pas de concentration déterminée mais ils sont fournis à une DO de 1.0 à 520nm,
2. la concentration en anticorps est comprise entre 5 et 10 µg/ml,
3. Une particule d'or de 10nm possède entre 7 et 10 molécules d'anticorps à sa surface,
4. la dilution recommandée est de 1/20.

Read More

Guillaume Boucher

Abcam Scientific Support

Answered on Jun 18 2013

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