Donkey Anti-Rabbit IgG H&L (ab182020)
Key features and details
- Donkey Anti-Rabbit IgG H&L
- Host species: Donkey
- Isotype: IgG
- Suitable for: ELISA, WB, IHC-P, IP
Overview
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Product name
Donkey Anti-Rabbit IgG H&L
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
The antibody reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY, less than 2%. -
Tested applications
Suitable for: ELISA, WB, IHC-P, IPmore details -
Immunogen
The details of the immunogen for this antibody are not available.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150074)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175651)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175780)
- Donkey Anti-Rabbit IgG H&L (HRP) (ab205722)
- Donkey Anti-Rabbit IgG H&L (Biotin) (ab207999)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/20000 - 1/200000.
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| WB |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
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ELISA
1/20000 - 1/200000. |
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WB
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
Images
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ELISA - Donkey Anti-Rabbit IgG H&L (ab182020)
Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab182020 has not yet been referenced specifically in any publications.