Donkey Anti-Rabbit IgG H&L (ab6701)

Submit a review Q&A (1)References (5)

Key features and details

  • Donkey Anti-Rabbit IgG H&L
  • Host species: Donkey
  • Suitable for: WB, IHC-P, IHC-Fr, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IP, Immunodiffusion, Conjugation, ICC/IF, ELISAmore details

  • Immunogen

    Rabbit IgG whole molecule

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
ELISA
1/40000 - 1/160000.
Notes
WB
1/2000 - 1/10000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
ELISA
1/40000 - 1/160000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab6701? Please let us know so that we can cite the reference in this datasheet.

ab6701 has been referenced in 5 publications.

  • Li Z  et al. Screening and identification of Theileria annulata subtelomere-encoded variable secreted protein-950454 (SVSP454) interacting proteins from bovine B cells. Parasit Vectors 14:319 (2021). PubMed: 34116718
  • Lowe AR  et al. Importin-ß modulates the permeability of the nuclear pore complex in a Ran-dependent manner. Elife 4:N/A (2015). PubMed: 25748139
  • Oeschger FM  et al. Gene expression analysis of the embryonic subplate. Cereb Cortex 22:1343-59 (2012). PubMed: 21862448
  • Martin P  et al. Effect of prototypical inducers on ligand activated nuclear receptor regulated drug disposition genes in rodent hepatic and intestinal cells. Acta Pharmacol Sin 31:51-65 (2010). PubMed: 20048746
  • Martin P  et al. Comparison of the induction profile for drug disposition proteins by typical nuclear receptor activators in human hepatic and intestinal cells. Br J Pharmacol 153:805-19 (2008). PubMed: 18037906

Customer reviews and Q&As

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Question

Looking for unlabeled secondary antibodies, as well as isotype control antibodies in preparations lacking BSA. I'm not sure what "H&L" means, so I'm holding off on ordering ab6701. Thanks,

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Answer

Thank you for your enquiry. This Donkey anti Rabbit　IgG (H+L), binds to both the heavy and light chains of the IgG and will bind to the F( c) and F( ab)2 portions of the rabbit primary　antibody. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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