Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab7084)

Submit a review Q&A (2)References (3)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, WB, ELISA, Dot blot, IHC-Fr

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 25nm Gold 6nm HRP Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, ELISA, Dot blot, IHC-Frmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 50% Glycerol (glycerin, glycerine), 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/400 - 1/2000.
IHC-P
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ELISA
1/4000 - 1/16000.
Dot blot
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Notes
ICC/IF
1/400 - 1/2000.
IHC-P
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ELISA
1/4000 - 1/16000.
Dot blot
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab7084? Please let us know so that we can cite the reference in this datasheet.

ab7084 has been referenced in 3 publications.

  • van Wijk LM  et al. The RAD51-FFPE Test; Calibration of a Functional Homologous Recombination Deficiency Test on Diagnostic Endometrial and Ovarian Tumor Blocks. Cancers (Basel) 13:N/A (2021). PubMed: 34203855
  • Choi Y  et al. Preventive effects of indole-3-carbinol against alcohol-induced liver injury in mice via antioxidant, anti-inflammatory, and anti-apoptotic mechanisms: Role of gut-liver-adipose tissue axis. J Nutr Biochem 55:12-25 (2018). PubMed: 29331880
  • Peng EW  et al. Myocardial expression of heat shock protein 70i protects early postoperative right ventricular function in cyanotic tetralogy of Fallot. J Thorac Cardiovasc Surg 141:1184-91 (2011). PubMed: 21397265

Customer reviews and Q&As

Show All Reviews Q&A
Submit a question

1-2 of 2 Q&A

Question

ich bin an einer immunohistochemischen Färbung von humanem CD8 und humanem FoxP3 (in Mausgewebe) interessiert.



- Idealerweise mit einen rabbit anti CD-8 Primärantikörper

- Und FoxP3 mit Alkalischer Phosphatase (o.a.) gelabeltem Sekundärantikörper, um ggf. später ein doublestaining zu machen



Dazu hätte ich zwei Fragen: Welcher Unterschied besteht zwischen Antikörpern aus Überstanden bzw. aufgereinigten Antikörpern? Gibt es spezielle Vor-oder Nachteile bei den jeweiligen Methoden?



Würden Sie mir bitte ein Angebot mit jeweils passenden Sekundärantikörper machen?

Read More
Answer

Vielen Dank für Ihre Anfrage.

Ich habe Ihnen hier vier Antikörper herausgesucht, die wenig bzw. gar keine Kreuzreaktion mit dem Maus-Protein aufweisen, so dass Sie nur das humane Protein im Mausgewebe detektieren sollten.

Drei der vier Antikörper stammen aus dem Kaninchen, falls Sie also eine Doppelfärbung durchführen möchten, wurde ich Vorschlagen, einen der Rabbit Antikörper mit einem ungelabelten Sekundär- Antikörper zu blockieren, und dann den sekundären Antikörper mit einem tertiären Antikörper nachzuweisen: zb. im Falle der Kombination aus ab93278 und ab10563.

Dazu passen wuerden dann die folgenden Antikörper: ab6831 Chicken polyclonal Secondary Antibody to Rabbit IgG - H&L gefolgt von ab97142 Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alkaline Phosphatase), pre-adsorbed ; und ab7084 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed.

Oder aber Sie benutzen zwei primäre Antikörper aus zwei verschiedenen Species (ab2481 und ab4055).

Als Sekundär- Antikoerper kommen dann ab7084 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed; und ab6886 Donkey anti-Goat IgG H&L (AP) secondary antibody.

Bezüglich Ihrer Frage nach dem Aufreinigungsgrad: Der einzige Vorteil eines aufgereinigten Antikörpers ist es, dass Sie eine Isotopenkontrolle berechnen können, da Sie genau wissen, wie viel Antikörper Sie einsetzen. Dies ist aber eigentlich nur in der Flow Zytometrie von Interesse.


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Question

Customer would like to know the crossreactivity of these two antibodies with human IgG? The datasheet states taht there is a minimum cross-reaction. Can you provide some data? % of cross-reaction? He is looking for a secondary antibody which does not cross-react with human IgG at all.

Read More
Answer

Thank you for your phone call regarding cross-reactivity of ab7084 and ab7062. We have just received some more information and would like to pass it onto you: Our QC criteria are less than 1% cross-reactivity to those minimized serum proteins when the titer gives 0.5 OD to its own antigen. For example if 1:20,000 dilution of 610-705-124 showed 0.5 OD when it reacts with Mouse IgG. Then its cross reactivity to Human IgG at 1:20,000 has to be less than 0.005 OD. If you are looking for more specific secondary antibodies, we would perhaps suggest looking at the datasheets of the following products. These do NOT cross-react with human IgG. Anti-mouse-ALP: ab5871, ab5880, ab5888; Anti-rabbit-ALP: ab6121, ab6251, ab6258, ab6518. We hope this information will be useful for you. If you need anything further or any help then please let us know.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com