Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab97082)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, WB, IHC-P, ELISA
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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Tested applications
Suitable for: ICC, WB, IHC-P, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details -
Immunogen
Other Immunogen Type corresponding to Rabbit IgG.
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
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| WB |
1/2500 - 1/25000. (Chemiluminescent)
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| IHC-P |
1/100 - 1/1000.
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| ELISA |
1/2500 - 1/25000. (Primary ELISA)
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| Notes |
|---|
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ICC
Use at an assay dependent dilution. |
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WB
1/2500 - 1/25000. (Chemiluminescent) |
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IHC-P
1/100 - 1/1000. |
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ELISA
1/2500 - 1/25000. (Primary ELISA) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab97082 has been referenced in 3 publications.
- Matsuoka T et al. Inhibition of NLRP3 inflammasome by MCC950 improves the metabolic outcome of islet transplantation by suppressing IL-1ß and islet cellular death. Sci Rep 10:17920 (2020). PubMed: 33087823
- Rigon L et al. Targeting Brain Disease in MPSII: Preclinical Evaluation of IDS-Loaded PLGA Nanoparticles. Int J Mol Sci 20:N/A (2019). PubMed: 31022913
- Ligaba-Osena A et al. Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana). Sci Rep 7:17104 (2017). WB . PubMed: 29213132
