Donkey Anti-Rabbit IgG H&L (DyLight® 550) (ab96892)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 550)
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (DyLight® 550)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 550 replaces DyLight® 549. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/200.
|
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| ICC/IF |
1/50 - 1/500.
|
|
| IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
| Notes |
|---|
|
Flow Cyt
1/50 - 1/200. |
|
ICC/IF
1/50 - 1/500. |
|
IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
Images
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Donkey Anti-Rabbit IgG H&L (DyLight® 550) (ab96892)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (12)
ab96892 has been referenced in 12 publications.
- Bos R et al. Altered action potential waveform and shorter axonal initial segment in hiPSC-derived motor neurons with mutations in VRK1. Neurobiol Dis 164:105609 (2022). PubMed: 34990802
- Gonçalves AB et al. CEP78 functions downstream of CEP350 to control biogenesis of primary cilia by negatively regulating CP110 levels. Elife 10:N/A (2021). PubMed: 34259627
- Fan SJ et al. Glutamine deprivation alters the origin and function of cancer cell exosomes. EMBO J 39:e103009 (2020). PubMed: 32720716
- Zhang I et al. Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures. Cells 9:N/A (2020). PubMed: 33171886
- El-Bazzal L et al. Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1. Hum Mol Genet 28:2378-2394 (2019). PubMed: 31090908
