Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (ab96921)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed
- Conjugation: DyLight® 594. Ex: 593nm, Em: 618nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
Conjugation
DyLight® 594. Ex: 593nm, Em: 618nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/50 - 1/200.
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
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Notes |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
Images
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Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (ab96921)
E14 mouse brain sample was stained with primary antibody Rabbit anti-Clim1. The secondary antibody used was ab96921 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.
This image is courtesy of an anonymous Abreview
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab96921 has been referenced in 18 publications.
- Liu J et al. Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis. Autophagy 18:971-989 (2022). PubMed: 34524943
- Xiu H et al. Neddylation Alleviates Methicillin-Resistant Staphylococcus aureus Infection by Inducing Macrophage Reactive Oxygen Species Production. J Immunol 207:296-307 (2021). PubMed: 34183370
- Rajendran A et al. Tissue-specific deletion of mouse basolateral uniporter LAT4 (Slc43a2) reveals its crucial role in small intestine and kidney amino acid transport. J Physiol 598:5109-5132 (2020). PubMed: 32841365
- Dolgodilina E et al. Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance. Fluids Barriers CNS 17:17 (2020). PubMed: 32046769
- Oparija-Rogenmozere L et al. Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteins. PLoS One 15:e0233863 (2020). PubMed: 32470053