Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50 - 1/500.
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| IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
|
| Flow Cyt |
1/50 - 1/200.
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| Notes |
|---|
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ICC/IF
1/50 - 1/500. |
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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Flow Cyt
1/50 - 1/200. |
Images
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Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (12)
ab96922 has been referenced in 12 publications.
- Hamade H et al. BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis. Front Immunol 13:841065 (2022). PubMed: 35812447
- Moser B et al. The inflammatory kinase IKKa phosphorylates and stabilizes c-Myc and enhances its activity. Mol Cancer 20:16 (2021). PubMed: 33461590
- Neddens J et al. Correlation of pyroglutamate amyloid ß and ptau Ser202/Thr205 levels in Alzheimer's disease and related murine models. PLoS One 15:e0235543 (2020). PubMed: 32645028
- Tan EE et al. Dominant-negative NFKBIA mutation promotes IL-1ß production causing hepatic disease with severe immunodeficiency. J Clin Invest 130:5817-5832 (2020). PubMed: 32750042
- Neddens J et al. Constant Levels of Tau Phosphorylation in the Brain of htau Mice. Front Mol Neurosci 13:136 (2020). PubMed: 32982685
