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    products/secondary-antibodies/donkey-rabbit-igg-hl-irdye-680rd-preadsorbed-ab216779.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Western blot - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)
  • Spectrum - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
  • Conjugation: IRDye® 680RD
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: WB, In-Cell ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm Gold 6nm HRP Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Tested applications

    Suitable for: WB, In-Cell ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit IgG. Whole molecule.

  • Conjugation

    IRDye® 680RD

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Affinity chromatography purified using antigens coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/25000.
In-Cell ELISA
1/200 - 1/800.
Notes
WB
1/5000 - 1/25000.
In-Cell ELISA
1/200 - 1/800.

Images

  • Western blot - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)
    Western blot - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)
    Lanes 1-3 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution (Anti-GAPDH antibody [6C5])
    Lane 4 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : NF-?B p60 knockout HAP1 cell lysate (20 µg)
    Lane 3 : HeLa cell lysate (20 µg)
    Lane 4 : A431 cell lysate (20 µg)

    Secondary
    Lane 1 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216779)
    Lanes 2-4 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD))


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab140751 overnight at 4°C. Antibody binding was detected using the Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216779 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Spectrum - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)
    Spectrum - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)

    Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab216779? Please let us know so that we can cite the reference in this datasheet.

ab216779 has been referenced in 1 publication.

  • Budden T  et al. Ultraviolet light-induced collagen degradation inhibits melanoma invasion. Nat Commun 12:2742 (2021). PubMed: 33980846

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