Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)
Key features and details
- Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
- Conjugation: IRDye® 680RD
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: WB, In-Cell ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster more details -
Immunogen
Full length native protein (purified) corresponding to Rabbit IgG. Whole molecule.
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Conjugation
IRDye® 680RD
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified using antigens coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/25000.
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| In-Cell ELISA |
1/200 - 1/800.
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| Notes |
|---|
|
WB
1/5000 - 1/25000. |
|
In-Cell ELISA
1/200 - 1/800. |
Images
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Western blot - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)Lanes 1-3 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution (Anti-GAPDH antibody [6C5])
Lane 4 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : NF-?B p60 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Lane 4 : A431 cell lysate (20 µg)
Secondary
Lane 1 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216779)
Lanes 2-4 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab140751 overnight at 4°C. Antibody binding was detected using the Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216779 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Spectrum - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab216779 has been referenced in 1 publication.
- Budden T et al. Ultraviolet light-induced collagen degradation inhibits melanoma invasion. Nat Commun 12:2742 (2021). PubMed: 33980846