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  1. Link

    products/secondary-antibodies/donkey-rabbit-igg-hl-preadsorbed-ab97081.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081)

  • Datasheet
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Key features and details

  • Donkey Anti-Rabbit IgG H&L preadsorbed
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, WB, ELISA

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

     

  • Tested applications

    Suitable for: ICC, IHC-P, WB, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Human, Mouse, Pig, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Other Immunogen Type corresponding to Rabbit IgG.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads .
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Unconjugated

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
WB
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000)
ELISA
1/100 - 1/10000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/10000)
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
WB
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000)
ELISA
1/100 - 1/10000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/10000)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab97081? Please let us know so that we can cite the reference in this datasheet.

ab97081 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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