Donkey Anti-Rabbit IgG H&L (TRITC) (ab6799)
Key features and details
- Donkey Anti-Rabbit IgG H&L (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Donkey
- Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, ELISA
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (TRITC)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
Immunogen
Rabbit IgG, whole molecule
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Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
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| Flow Cyt |
Use at an assay dependent dilution.
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| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| ELISA |
1/10000 - 1/50000.
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| Notes |
|---|
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ICC/IF
1/1000 - 1/5000. |
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Flow Cyt
Use at an assay dependent dilution. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab6799 has been referenced in 9 publications.
- Wu J et al. Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3-E1 and MDPC23 by targeting COL15A1. Mol Med Rep 22:1737-1746 (2020). PubMed: 32582985
- Yin L et al. The family of apoptosis-stimulating proteins of p53 is dysregulated in colorectal cancer patients. Oncol Lett 15:6409-6417 (2018). PubMed: 29731851
- Guo HY & Cui ZJ Extracellular Histones Activate Plasma Membrane Toll-Like Receptor 9 to Trigger Calcium Oscillations in Rat Pancreatic Acinar Tumor Cell AR4-2J. Cells 8:N/A (2018). PubMed: 30577532
- Tao X et al. In vitro examination of microglia-neuron crosstalk with BV2 cells, and primary cultures of glia and hypothalamic neurons. Heliyon 4:e00730 (2018). PubMed: 30148218
- Li Y et al. Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways. Biomed Res Int 2018:3242574 (2018). PubMed: 29850504
- Li Y et al. Apelin induces the proliferation, migration and expression of cytoskeleton and tight junction proteins in human RPE cells via PI-3K/Akt and MAPK/Erk signaling pathways. Int J Clin Exp Pathol 10:10711-10729 (2017). PubMed: 31966415
- Song C et al. Aminated nanomicelles as a designer vaccine adjuvant to trigger inflammasomes and multiple arms of the innate immune response in lymph nodes. Int J Nanomedicine 12:7501-7517 (2017). IF . PubMed: 29066896
- Guo Z et al. E-cadherin interactome complexity and robustness resolved by quantitative proteomics. Sci Signal 7:rs7 (2014). PubMed: 25468996
- Liang G et al. Serum Response Factor Controls CYLD Expression via MAPK Signaling Pathway. PLoS One 6:e19613 (2011). ICC/IF . PubMed: 21573132