Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)

    Key features and details

    • Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed
    • Conjugation: TRITC. Ex: 547nm, Em: 572nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

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    Overview

    • Product name

      Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed
      See all IgG secondary antibodies

    • Host species

      Donkey

    • Target species

      Rabbit

    • Tested applications

      Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

    • Minimal
      cross-reactivity


      Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

    • Immunogen

      Full length native Rabbit IgG (purified).

    • Conjugation

      TRITC. Ex: 547nm, Em: 572nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG

    • Conjugation notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7080 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Immunomicroscopy
    Flow Cyt
    IHC-P
    IHC-Fr
    ELISA
    Notes
    Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Images

    • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)
      Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)

      ab7080 was used at dilution 1/200 with the primary antibody ab2900 in ICC/IF. See the review on ab2900.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab7080? Please let us know so that we can cite the reference in this datasheet.

    ab7080 has been referenced in 1 publication.

    • Zhang J  et al. Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis. Lipids Health Dis 19:39 (2020). PubMed: 32169116

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