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Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)

Submit a review Q&A (1)References (1)
Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)

    Key features and details

    • Donkey Anti-Sheep IgG H&L (DyLight® 550)
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 650 FITC Gold 12nm HRP Texas Red ® TRITC Unconjugated

    Overview

    • Product name

      Donkey Anti-Sheep IgG H&L (DyLight® 550)
      See all IgG secondary antibodies

    • Host species

      Donkey

    • Target species

      Sheep

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

    • Tested applications

      Suitable for: IHC-P, ICC/IF, Flow Cytmore details

    • Conjugation

      DyLight® 550. Ex: 562nm, Em: 576nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • General notes

      DyLight® 550 replaces DyLight® 549.

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96940 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    Images

    • Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)
      Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab96940? Please let us know so that we can cite the reference in this datasheet.

    ab96940 has been referenced in 1 publication.

    • Mikedis MM & Downs KM Widespread but tissue-specific patterns of interferon-induced transmembrane protein 3 (IFITM3, FRAGILIS, MIL-1) in the mouse gastrula. Gene Expr Patterns 13:225-39 (2013). PubMed: 23639725

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hi,

    I have reviewed this profile. I might not clarify clearly what I need. I need to have the spectrum of the dye as you may know the excitation spectrum may cover a certain range not only at a certain point. I am doing multiple staining of different proteins so the overlap of either excitation or emission affect the result a lot. Can you please give me more details of your dye? (such as dye light). Thanks a lot!

    all the best

    Read More
    Answer

    Thank you for your reply. My colleague is not in the office today and that is the reason I am writing to you.

    I ma sorry there was a misunderstanding.

    We do have information on the spectra of DyLight® fluorochromes on our homepage:

    https://www.abcam.com/index.html?pageconfig=resource&rid=12934

    The spectra can be reviewed on the bottom of the page. Unfortunately, we do not have a spectra viewer where multiple dyes can be combined and tested for overlapping. I am sorry if this causes any inconvenience.

    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Maybe it is worth checking their website for a spectra viewer also.

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

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