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Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

Submit a review Q&A (2)References (22)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
  • Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
  • ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

Key features and details

  • Donkey Anti-Sheep IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 650 FITC Gold 12nm Texas Red ® TRITC Unconjugated

Overview

  • Product name

    Donkey Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Sheep

  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details

  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.50
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/5000.
IHC-Fr
1/1000 - 1/5000.
IHC-P
1/1000 - 1/5000.
WB
1/3000 - 1/15000.
ELISA
1/150000.
Immunomicroscopy
Use at an assay dependent dilution.
Dot blot
Use at an assay dependent dilution.
Notes
ICC/IF
1/1000 - 1/5000.
IHC-Fr
1/1000 - 1/5000.
IHC-P
1/1000 - 1/5000.
WB
1/3000 - 1/15000.
ELISA
1/150000.
Immunomicroscopy
Use at an assay dependent dilution.
Dot blot
Use at an assay dependent dilution.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

    IHC image of Histone H3 staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab128012, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6900, 1/1000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
    Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

    ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

  • ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
    ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

    ELISA results of purified ab6900 tested against purified Sheep IgG.

    Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (22)

Publishing research using ab6900? Please let us know so that we can cite the reference in this datasheet.

ab6900 has been referenced in 22 publications.

  • Liang Y  et al. Monomeric C-reactive protein level is associated with osteoarthritis. Exp Ther Med 23:277 (2022). PubMed: 35317443
  • Koorneef LL  et al. Dexamethasone-associated metabolic effects in male mice are partially caused by depletion of endogenous corticosterone. Front Endocrinol (Lausanne) 13:960279 (2022). PubMed: 36034417
  • Tomida S  et al. The stem region of α1,6-fucosyltransferase FUT8 is required for multimer formation but not catalytic activity. J Biol Chem 298:102676 (2022). PubMed: 36336076
  • Cui H  et al. Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy. J Cell Mol Med 25:9634-9646 (2021). PubMed: 34486218
  • Shimizu M  et al. Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression. Cancers (Basel) 13:N/A (2021). PubMed: 34572779
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Hi,
I recently purchased a Secondary Antibody to Sheep IgG - H&L (HRP)
(ab6900). It was delivered today but the solution looks cloudy and and
there are clearly visible particles floating in it. I have never bought
this particular product before, but I have never seen an antibody
solution like this before and am worried about the quality of the
antibody. I itend to use the antibody in a 3 day experiment and really
want to avoid wasting time and money on it. The batch number is
GR54072-1.
the order reference number is 1016507 and the purchase Order Number
462EWD. Would you mind having a look at this batch and sending a
replacement product, if possible?
Thanks,

Read More
Answer

Thank you for contacting us.

Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest pipetting up and down few times,vortexing the vial and using this antibody as normal. In our retesting experiment and from customer’s feedback we can say that ab is absolutely fine to use. So I would really encourage you to use the antibody first; If in case the antibody fails please do not hesitate to contact me for further assistance.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Kære Abcam,
We have today received the above order - 1 x 1 Donkey Polyclonal Secondary Antibody to Sheep IgG - H&L (HRP)(Lot no. GR54072).
Unfortunately the antibody contained large precipitations which did not dissolve itself after mixing or after having been warmed-up to 37 degrees for 2-15 min. Please see the picture attached.
Please let us have a new antibody but please be sure that the new antibody is an antibody which is of good quality.
Do you want this one returned for some sort of examination ?

best regards







Med venlig hilsen/Best regards




________________________________

Read More
Answer

Thank you for your inquiry.

It is normal to have brown precipitate form for HRP conjugates. The antibody that remains in solution is functional and the brown pellet should be discarded.

Irecommend to spin down the vial and try the antibody from the supernatantin your experiment. You might want to increase the amount you are using a little bit.

I would like to assureyou that if one of our products is not functioning as stated on the datasheet and was purchased with the last six month we will be happy to provide a free of charge replacement of credit note.

Please let me know if the antibody does not generate successful results after removing the precipitate.

Thank you for your cooperation.

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