Goat Anti-Chicken IgY H&L (Agarose) (ab76444)
Key features and details
- Goat Anti-Chicken IgY H&L (Agarose)
- Conjugation: Agarose
- Host species: Goat
- Isotype: IgG
- Suitable for: IP
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DyLight® 488
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FITC
Gold 10nm
Gold 25nm
HRP
HRP
PE
Texas Red ®
Unconjugated
Unconjugated
Overview
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Product name
Goat Anti-Chicken IgY H&L (Agarose)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Specificity
Ab76444 is specific to chicken IgY and will not cross react with mammalian IgG. -
Tested applications
Suitable for: IPmore details -
Immunogen
Purified chicken IgY
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Conjugation
Agarose
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Goat anti-chicken IgY antibodies were affinity purified from the sera over a chicken IgY column. -
Conjugation notes
Affinity purified Goat anti-chicken IgY antibodies were conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50 mM HEPES buffer (pH 7.6) with 5 mM EDTA (“TE buffer”). Sodium azide was then added to a final concentration of 0.02% (w/v). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Binding Capacity is 1.0 mg of Chicken IgY per ml bed volume. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
IP
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab76444 has been referenced in 1 publication.
- Oliveira R et al. The novel ECF56 SigG1-RsfG system modulates morphological differentiation and metal-ion homeostasis in Streptomyces tsukubaensis. Sci Rep 10:21728 (2020). PubMed: 33303917
